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(一)心肌上的輔酶Ⅰ細胞色素c還原酶系活力的最適pH和水溶性輔酶Ⅰ細胞色素c還原酶活力的最適pH顯著不同,酶系物理狀態對酶活力影響頗大。 (二)水溶性輔酶Ⅰ細胞色素c還原酶不能和心肌製劑顆粒上的細胞色素c作用。心肌製劑在經過[2,3]二氫硫基丙醇處理完全破壞原有輔酶Ⅰ細胞色素c還原酶系活力以後仍能抽提出活力很強的水溶性輔酶Ⅰ細胞色素c還原酶。這些以及我們過去曾經討論過的一些事實都說明Mahler等所獲得的水溶性輔酶Ⅰ细胞色素C還原酶是一個矯作物。 (三)本研究指出根據用人為的方法從複雜酶系中抽出的酶的性質簡單地判斷該酶在整個酶系中的作用不一定是可靠的。
(1) The optimal pH of coenzyme I cytochrome c reductase activity in myocardium is significantly different from that of water-soluble coenzyme I cytochrome c reductase activity, and the physical state of enzyme has a great influence on enzyme activity. (B) water-soluble coenzyme I cytochrome c reductase and myocardial agent particles on the role of cytochrome c. Myocardial preparations can still extract the highly active water-soluble coenzyme I cytochrome c reductase after the [2,3] dihydrothiophenol treatment completely destroys the original coenzyme I cytochrome c reductase activity. These and some of the facts we have discussed in the past suggest that water-soluble coenzyme I cytochrome C reductase obtained by Mahler et al. Is an artifact. (C) The present study indicates that simply determining the role of this enzyme in the entire enzyme system is not necessarily reliable, based on the nature of the enzyme extracted from the complex enzyme system by an artificial approach.