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人脐静脉内皮细胞在体外用~(60)Coγ线照射,剂量从0至50Gy。辐照后早期内皮细胞培养液中t—PA抗原和活性都迅速增高,PAI活性降低。辐照6天后t—PA抗原和活性都随着辐照剂量的增加而降低,PAI活性增高。此外,辐照后早期内皮细胞血栓调节素、PGI_2和vWF释放都增加;但辐照6天后内皮细胞合成血栓调节素和PGI_2能力降低,而合成vWF增加。结果表明内皮细胞经的~(60)Coγ线照射后早期纤溶和抗凝功能都亢进;后期则纤溶和抗凝功能都低下,促凝活性增高。对辐照后内皮细胞纤溶、抗凝功能的改变与急性辐射病的发病机理作了讨论。
Human umbilical vein endothelial cells in vitro with ~ (60) Co gamma ray irradiation, the dose from 0 to 50Gy. In the early stage of irradiation, the t-PA antigen and activity in endothelial cell culture fluid increased rapidly, and PAI activity decreased. After 6 days of irradiation, t-PA antigen and activity decreased with the increase of radiation dose, PAI activity increased. In addition, the release of endothelial cells thrombomodulin, PGI_2 and vWF increased early after irradiation; however, the ability of endothelial cells to synthesize thrombomodulin and PGI_2 decreased and the synthesis of vWF increased after 6 days of irradiation. The results showed that the early fibrinolysis and anticoagulant function of endothelial cells were accelerated by ~ (60) Coγ ray irradiation. In the latter stage, the fibrinolytic and anticoagulant functions were low and the procoagulant activity increased. The fibrinolytic and anticoagulant changes of endothelial cells after irradiation were discussed with the pathogenesis of acute radiation sickness.