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目的:探讨益精调气中药复方制剂在抑制左旋多巴神经毒性中的作用。方法:将40只大鼠随机分为对照组和实验组各20只,对照组大鼠采用常规方法治疗,实验组大鼠采用益精调气中药复方治疗,采用细胞凋亡、行为学、Niss染色以及免疫组织化学方法检测治疗10天、12天、14天时大鼠黑质细胞凋亡数、旋转行为及bFGF蛋白水平。结果:实验组大鼠12天与14天灌胃时间旋转行为均显著高于对照组,差异有统计学意义(P<0.05);实验完毕后将大鼠处死,大鼠14天时黑质细胞凋亡数、bFGF蛋白表达显著多于对照组,差异有统计学意义(P<0.05)。结论:益精调气中药复方制剂治疗帕金森大鼠效果理想,能够减轻左旋多巴神经毒性作用。
Objective: To explore the effect of Yijingqiqiqi compound prescription on the inhibition of levodopa neurotoxicity. Methods: Forty rats were randomly divided into control group and experimental group, 20 rats in each group. The rats in the control group were treated by conventional method. The rats in the experimental group were treated with Yijingqiqi compound. The apoptosis, Staining and immunohistochemistry were used to detect the number of apoptotic cells, the rotation behavior and the bFGF protein level in rat substantia nigra at 10 days, 12 days and 14 days after treatment. Results: The rats in the experimental group at 12 and 14 days after gavage were significantly higher in rotation than those in the control group (P <0.05). After the experiment, the rats were sacrificed, The number of bFGF protein expression was significantly higher than that of the control group, with significant difference (P <0.05). Conclusion: Yijingjingqi compound compound is effective in treating Parkinson ’s disease and can relieve the neurotoxic effect of levodopa.