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目的:观察灯盏花素对肿瘤诱导的小鼠免疫抑制的影响。方法:小鼠脾脏细胞体外培养观察灯盏花素对淋巴细胞增殖和淋巴细胞因子分泌的的影响;lewis肺癌荷瘤小鼠迟发型变态反应、血清淋巴细胞因子水平及CD8+T细胞对lewis肺癌的细胞毒作用检测灯盏花素对小鼠免疫抑制的逆转作用。结果:灯盏花素体外对刀豆蛋白(ConA)诱导的小鼠脾脏细胞增殖有增强作用,能抑制转化生长因子β1(TGF-β1)和白细胞介素4(IL-4),促进的干扰素λ(IFN-λ)和白细胞介素2(IL-2)。体内灯盏花素能逆转荷瘤小鼠迟发型变态反应、血清淋巴细胞因子水平及CD8+T细胞对lewis肺癌的无能作用,并一定程度抑制肿瘤生长。结论:灯盏花素作为TGF-β1抑制剂对肿瘤诱导的小鼠免疫抑制有逆转作用。
Objective: To observe the effect of scutellarin on tumor-induced immune suppression in mice. METHODS: Mouse spleen cells were cultured in vitro to observe the effect of breviscapine on lymphocyte proliferation and lymphocyte factor secretion; Lewis allergic reaction, serum lymphocyte factor levels and CD8+ T cells against lewis lung cancer in mice bearing Lewis lung cancer. Cytotoxicity was used to detect the reversal effect of erigeron on immunosuppression in mice. RESULTS: Breviscapine enhanced the proliferation of mouse spleen cells induced by ConA in vitro and inhibited transforming growth factor-β1 (TGF-β1), interleukin-4 (IL-4) and promoted interferon. Lambda (IFN-λ) and Interleukin 2 (IL-2). Breviscapine can reverse the late-type hypersensitivity, serum lymphocyte cytokines, and the inability of CD8+T cells to Lewis lung cancer in tumor-bearing mice, and inhibit tumor growth to some extent. Conclusion: Breviscapine as a TGF-β1 inhibitor has a reversal effect on tumor-induced immune suppression in mice.