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48 Wistar rats(190--250g) were used in this study.The pain threshold was very signifiantly hghier after lesar stimulation(LSA) than before LSA (P<0.001). Subslamce P like immunorcaction(SP-LI)was significantly enhanced (P<0.001) and enkephalin (ENK-LI) markedlydiminished (P<0.002) in the rat spinal cord of laser group than control group.Under transmissional electron microscope, the special dendroaxonic synapsewere found, the dendrite containi clusters of vesicles in a relatively lucentcytoplasm, but lacks a clearly defines ribosomes or endoplasmic reticulum. Thedouble labeling showed, we believe, direct contact between the ENK-LI contai-ning presynaptic dendrite and the SP-LI containing axonal terminal.
The results showed that the pain threshold was very signifiantly hghier after lesar stimulation (LSA) than before LSA (P <0.001). Subslamce P like immunorcaction (SP-LI) was significantly enhanced P <0.001) and enkephalin (ENK-LI) markedly diminished (P <0.002) in the rat spinal cord of laser group than control group .Under transmissional electron microscope, the special dendroaxonic synapsewere found, the dendrite containi clusters of vesicles in a relatively lucentcytoplasm , but lacks a clearly defined ribosomes or endoplasmic reticulum. The double labeling showed, we believe, direct contact between the ENK-LI contai-ning presynaptic dendrite and the SP-LI containing axonal terminals.