论文部分内容阅读
目的研究右旋糖酐-磁性层状复合氢氧化合物-氟尿嘧啶转运模型在大鼠体内药物分布的磁靶向性。方法建立高效液相色谱内标法,测定给药后大鼠体内各组织的氟尿嘧啶分布浓度;用紫外分光光度法、原子吸收法及等离子体发射光谱法测定给药干预后大鼠各组织铁的分布浓度,通过普鲁士蓝铁染色直观评价铁的组织分布差异。结果高效液相色谱内标法回收率高、精密度好;经腹腔注射给药后,背右侧及头部设立磁场部位的药物浓度明显高于其他远离磁场的组织部位,数值分布有统计学意义,证明三级复合模型具有理想的磁靶向运转效果。结论在外加磁场的作用下,右旋糖酐-磁性层状复合氢氧化合物-氟尿嘧啶转运模型能在靶向部位明显富集,表现良好的磁靶向性。
Objective To study the magnetic targeting of drug distribution in dextran-magnetic layered double hydroxide-fluorouracil transport model in rats. Methods The internal standard method of high performance liquid chromatography (HPLC) was established to determine the concentration of fluorouracil in each tissue of the rats after administration. The contents of iron in tissues of rats after administration were determined by ultraviolet spectrophotometry, atomic absorption spectrometry and plasma emission spectrometry Distribution of concentrations, through the Prussian blue-iron staining visual evaluation of the differences in tissue distribution of iron. Results High performance liquid chromatography with internal standard method had high recovery rate and good precision. After intraperitoneal injection, the concentration of drug in the right side of the back and the head was significantly higher than that in other tissues away from the magnetic field. The distribution of the values was statistically Significance, prove that the three-level composite model has the ideal magnetic target operation effect. Conclusion Under the applied magnetic field, the dextran-magnetic layered double hydroxide-fluorouracil transport model can be significantly enriched in the target site, showing good magnetic targeting.