血吸虫病肝纤维化实验动物模型的建立与评价

• 作 者: 何永康 周杰 喻鑫玲 刘宇 聂东宋 候循亚 孙渊 朱剑君 赵雅琴 李岳生
• 机 构: 湖南理工学院,湖南省血吸虫病防治所,
• 出 处: 热带医学杂志
• 发表日期: 2009年10期
• 关键词: 实验动物模型 血吸虫病 实验动物 肝纤维化模型 日本血吸虫 病理肝纤维化 肝纤维化程度 肝纤维化治疗 吡喹酮 肝组织病理学

目的建立及评估血吸虫病肝纤维化实验动物模型。方法以34只日本大耳白兔为实验动物,随机分为3组,即高剂量组(A组)、低剂量组(B组)及对照组(C组)建立肝纤维化模型,每只感染日本血吸虫尾蚴100条,感染后12～13周,高剂量组和低剂量组采用清肝口服液(DHT)进行干预治疗;感染后18周,按吡喹酮剂量300mg/kg体重对3组动物进行杀虫治疗,以病理学和血清HYP、HA水平作为模型评估指标。结果感染后第12～13周,肝纤维化模型形成,从病理学结果、生化指标均产生了不同程度的改变。A组、B组干预治疗后血清中的HYP/HA较干预前明显降低(P<0.01);肝组织病理学结果显示:肝纤维化程度依次为高剂量组<低剂量组<对照组(P<0.01),且血清HYP、HA含量随着病理肝纤维化程度增高而升高。结论检测血清HYP和HA水平是评价和考核肝纤维化治疗效果的简便、快速、可靠、又无损伤的指标,而且与病理学检查结果相符,具有实际应用价值。 Objective To establish and evaluate the animal model of liver fibrosis induced by schistosomiasis. Methods Thirty-four Japanese white rabbits were used as experimental animals and randomly divided into three groups: high dose group (A group), low dose group (B group) and control group (C group) Infection of Schistosoma japonicum cercariae 100, 12 to 13 weeks after infection, high-dose group and low-dose group with Qinggan oral liquid (DHT) for intervention; 18 weeks after infection, praziquantel dose of 300mg / kg body weight of three groups The animals were treated with insecticide, pathological and serum HYP, HA levels as a model to assess indicators. Results From the 12th to the 13th week after infection, the hepatic fibrosis model was formed, and the pathological changes and biochemical indexes were changed to some extent. The serum levels of HYP / HA in group A and group B were significantly lower than those before intervention (P <0.01). The pathological results of liver histopathology showed that the levels of liver fibrosis were high dose group, low dose group