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目的通过检测中国仓鼠卵巢(CHO)细胞中过氧化氢酶(CAT)活力和丙二醛(MDA)含量的变化研究微囊藻毒素-LR(MC-LR)对CHO细胞的氧化应激作用。方法调整CHO细胞密度约为1×105/ml,分别用终浓度为0(对照)、1、5、10、15μg/ml的MC-LR染毒24 h后,采用MTT法测细胞活性,计算半致死效应浓度(EC50)。调整细胞密度约为1×106/ml,分别用终浓度为0(对照)、2.5(1/4 EC50)、5(1/2 EC50)、10μg/m(lEC50)的MC-LR染毒24 h后,测定细胞中CAT活力和MDA含量。结果随着MC-LR染毒浓度的升高,CHO细胞活性呈下降趋势。与对照组相比,1μg/ml染毒组细胞活性降低,但差异无统计学意义;5、10、15μg/ml MC-LR染毒组CHO细胞活性较低,差异均有统计学意义(P<0.05),且10μg/ml MC-LR染毒组细胞活性为53.8%,接近50%,故EC50约为10μg/ml。与对照组相比,各浓度MC-LR染毒组CHO细胞内CAT活力较低,MDA含量较高,差异均有统计学意义(P<0.05)。结论 MC-LR染毒可使CHO细胞中CAT活力降低,MDA含量升高,提示其对CHO细胞有氧化损伤作用。
OBJECTIVE: To investigate the effects of microcystin-LR (MC-LR) on oxidative stress in CHO cells by detecting the activity of catalase (CAT) and the content of malondialdehyde (MDA) in Chinese hamster ovary (CHO) Methods The density of CHO cells was adjusted to about 1 × 10 5 / ml, and the cell viability was measured by MTT assay after the cells were treated with MC-LR (final concentration of 0, control), 1,5,10 and 15 μg / Semi-lethal effect concentration (EC50). The cell density was adjusted to about 1 × 10 6 cells / ml and MC-LR was exposed to a final concentration of 0 (control), 2.5 (1/4 EC50), 5 (1/2 EC50), 10 μg / h after the determination of cell activity and MDA content of CAT. Results As the concentration of MC-LR increased, the activity of CHO cells showed a decreasing trend. Compared with the control group, the activity of cells in the 1μg / ml treatment group was decreased, but the difference was not statistically significant. The activities of CHO cells in the 5,10,15μg / ml MC-LR group were lower than that in the control group (P <0.05). The cell viability of MC-LR treated with 10μg / ml of MC-LR was 53.8%, which was close to 50%, so the EC50 was about 10μg / ml. Compared with the control group, the CAT activity and the content of MDA in MC cells were lower than those in the control group (P <0.05). Conclusion MC-LR could decrease the activity of CAT and increase the content of MDA in CHO cells, suggesting that it could oxidatively damage CHO cells.