论文部分内容阅读
目的了解猕猴、鼠、旱獭、鸽子、鸡、鱼、蟾蜍血清中抗-HEV抗体流行情况。方法应用双抗原夹心酶联免疫法检测猕猴、鼠、旱獭、鸽子、鸡、鱼和蟾蜍血清中抗-HEV抗体。结果总抗-HEV抗体阳性率为14.41%(113/784)。7种动物中猕猴血清抗-HEV抗体阳性率为7.26%(9/124);鼠和旱獭抗-HEV抗体阳性率分别为34.91%(59/169)和4.74%(9/190);鱼和蟾蜍分别为17.33%(13/75)和25.56%(23/90);鸡和鸽子血清中未检测到抗-HEV抗体。结论猕猴、鼠、旱獭、鱼和蟾蜍中存在HEV感染,鼠抗-HEV抗体阳性率最高;其次为蟾蜍和鱼;鸡和鸽子中未检测到抗-HEV抗体。
Objective To investigate the prevalence of anti-HEV antibodies in the serum of macaque, mouse, marmot, pigeon, chicken, fish and toad. Methods The anti-HEV antibodies in sera of monkeys, rats, marmot, pigeon, chicken, fish and toad were detected by double antigen sandwich enzyme-linked immunosorbent assay. Results The positive rate of total anti-HEV antibody was 14.41% (113/784). The positive rate of anti-HEV antibody in cynomolgus monkeys was 7.26% (9/124) in 7 kinds of animals and 34.91% (59/169) and 4.74% (9/190) in mice and marmots, respectively Toad was 17.33% (13/75) and 25.56% (23/90), respectively. No anti-HEV antibodies were detected in the serum of chickens and pigeons. Conclusion HEV infection is present in rhesus macaque, mouse, marmot, fish and toad. The positive rate of anti-HEV antibody is the highest in mice, followed by toad and fish. No anti-HEV antibodies were detected in chicken and pigeon.