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自1948年,Peter,Medawar开始构想运用离体组织培养作为实验材料以来,1960年Cruicksha-nk和Corper、1975年Rheinwald和Green分别先后培养成功了哺乳动物和人的皮肤细胞.随着培养条件和技术的不断改进近几年实验方法趋于标准化,皮肤细胞原代培养技术首先被运用于
Since 1948, when Peter and Medawar started to think about the use of ex vivo tissue culture as experimental material, mammalian and human skin cells were successively cultivated by Cruickshain and Corper in 1960 and Rheinwald and Green in 1975 respectively. With the culture conditions and Continuous improvement of technology Experimental methods tend to be standardized in recent years, primary skin cell culture technology was first applied to