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所用水稻叶色突变体为自然突变,并命名为白淮稻7号,其叶色表型为绿-白-绿,且突变表型只有在移栽等因素引起的机械损伤信号胁迫下才会产生。研究结果表明,叶色转白前,突变体生长态势、叶色、叶绿素a含量和叶绿体超显微结构与野生型差异不大;叶色转白后,突变体总叶绿素、叶绿素a、叶绿素b和类胡萝卜素含量都显著低于野生型和叶色转白前,而叶绿体中的类囊体逐渐降解,基粒片层减少、基粒数量明显减少,且在成熟后突变体叶色黄化、植株变矮小。遗传分析表明,突变性状由1对隐性核基因控制。以该突变体与江西1587的F2群体为定位群体,将突变基因定位于水稻第11染色体分子标记L59.2-7和L64.8-11之间大约740.5kb的区间内。认为该突变基因是一个新的水稻叶色突变基因,暂命名为GWGL。
The rice leaf color mutant was a natural mutation named Baihuaiyao 7 and its leaf color phenotype was green-white-green, and the mutant phenotype was only under the stress of mechanical damage caused by transplanting and other factors produce. The results showed that the growth trend, leaf color, chlorophyll-a content and chloroplast ultrastructure of the mutants were not significantly different from those of the wild type before the leaves turned white, and the total chlorophyll, chlorophyll a, chlorophyll b And carotenoid content were significantly lower than before the wild-type and leaf color turned white, while the thylakoid gradual degradation of chloroplast, the basement layer reduced, the number of the base reduced significantly, and the mutant leaf color after ripening , Plants become shorter. Genetic analysis showed that the mutant trait was controlled by a pair of recessive nuclear genes. The F2 population of this mutant and Jiangxi 1587 were used as the locating group, and the mutant gene was located in the interval of about 740.5kb between rice chromosome markers L59.2-7 and L64.8-11. The mutant gene is considered as a new rice leaf color mutant gene and tentatively named GWGL.