论文部分内容阅读
目的:通过动物实验观察ω-3脂肪乳剂单用及联合表阿霉素(EPI)对胃癌移植瘤和荷瘤裸鼠的作用。方法:以胃癌细胞株MGC-803建立的裸鼠动物模型为研究对象,采用ω-3脂肪乳剂单用灌胃及联合表阿霉素腹腔注射的干预手段。荷瘤鼠随机分为对照(A)组、ω-3脂肪乳剂灌胃(B)组、EPI腹腔注射(C)组及ω-3脂肪乳剂联合EPI(D)组。观察荷瘤鼠的一般状况、体重变化、肿瘤生长、转移情况;光镜观察胃癌移植瘤的改变;电镜观察胃癌移植瘤超微结构的改变。结果:①D组肿瘤明显小于A、B组(P<0.05);D组肿瘤的生长速度和大小明显小于C组(P<0.05),A、B组间肿瘤生长无明显差异(P>0.05);与A组相比,B、C、D组未见粘连及淋巴结转移,肿瘤坏死灶较多。②C、D组均出现化疗药物的毒副作用,甚至死亡,但D组状况明显好于C组。③.B组肿瘤组织在电镜下可见肿瘤细胞变性,D组标本中可见到凋亡细胞。结论:①ω-3脂肪酸单独应用可减少胃癌肿瘤的侵袭性和局部转移,导致肿瘤组织局灶性坏死,并能引起肿瘤细胞代谢障碍、凋亡或死亡。②ω-3脂肪酸可增强EPI抗肿瘤效果,说明与EPI单独作用相比,联用ω-3脂肪酸可减少EPI用量。③ω-3脂肪酸可改善化疗动物的一般情况、减少EPI的毒副作用,而对正常器官无不良影响,对机体是安全的。
OBJECTIVE: To observe the effect of omega-3 lipid emulsion combined with epirubicin (EPI) on gastric cancer xenografts and tumor-bearing nude mice through animal experiments. Methods: The animal model of nude mice established by gastric cancer cell line MGC-803 was used as the research object. Oral administration of ω-3 fat emulsion and intraperitoneal injection of epirubicin alone were used. Tumor bearing mice were randomly divided into control group (A), omega-3 lipid emulsion group (B), EPI intraperitoneal injection (group C) and omega-3 fatty emulsion combined with EPI (D) group. Observe the general condition of tumor-bearing mice, body weight changes, tumor growth and metastasis; observe the changes of the tumor xenografts with light microscopy; and observe the ultrastructural changes of the transplanted gastric cancer by electron microscope. Results: ① The tumor size in group D was significantly lower than that in group A and B (P <0.05). The growth rate and size of group D were significantly lower than that in group C (P <0.05), but there was no significant difference between group A and group B (P> 0.05) Compared with group A, there was no adhesion and lymph node metastasis in group B, C and D, and tumor necrosis was more. ②C and D groups all had the side effects of chemotherapy drugs and even died, but the condition of group D was obviously better than that of group C. ③ tumor tissue in group B. The tumor cell degeneration can be seen under electron microscope, apoptotic cells can be seen in group D specimens. Conclusion: ①ω-3 fatty acid alone can reduce the invasiveness and local metastasis of gastric cancer, leading to focal necrosis of tumor tissue and causing metabolic disorders, apoptosis or death of tumor cells. ②ω-3 fatty acids can enhance the anti-tumor effect of EPI, indicating that compared with the EPI alone, combined with omega-3 fatty acids can reduce the amount of EPI. ③ ω-3 fatty acids can improve the general situation of chemotherapy animals, reduce the side effects of EPI, but no adverse effects on normal organs, the body is safe.