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为探索肺内调节肽血管活性肠肽（ＶＩＰ） 和表皮生长因子（ＥＧＦ） 抗氧化保护的基因机制， 用逆转录聚合酶链式反应（ＲＴＰＣＲ） 及Ｓｏｕｔｈｅｒｎ ｂｌｏｔ 杂交等方法检测原代培养的兔支气管上皮细胞（ＢＥＣ） 内ｂｃｌ２ 和ｃｍｙｃ 基因的表达情况， 观察ＶＩＰ、ＥＧＦ及热应激对这两个基因表达的影响。结果显示：（１） 基础情况下ＢＥＣ内有ｂｃｌ２ 和ｃｍｙｃ 基因的低水平表达；（２） ＥＧＦ和ＶＩＰ均明显增强ｂｃｌ２ 和ｃｍｙｃ 的转录，ＥＧＦ的作用更强， 而热应激无明显效应；（３） ｂｃｌ２ 和ｃｍｙｃ 两者的转录有显著相关性。上述结果提示，ＶＩＰ和ＥＧＦ等肺内调节肽可通过上调ｂｃｌ２ 基因表达增强支气管上皮细胞的抗氧化能力；ｃｍｙｃ 基因的编码产物可能是ｂｃｌ２ 基因转录的上游调节因子。
In order to explore the genetic mechanism of antioxidative protection of vasoactive intestinal peptide (VIP) and epidermal growth factor (EGF) in lung, the primary culture was detected by reverse transcriptase-polymerase chain reaction (RTPCR) and Southern blot hybridization Of rabbit bronchial epithelial cells (BEC) bcl 2 and c myc gene expression, to observe the VIP, EGF and heat stress on the expression of these two genes. The results showed that: (1) There was a low level expression of bcl-2 and c-myc genes in BEC in the basal condition; (2) Both EGF and VIP significantly enhanced the transcription of bcl-2 and c-myc, While no significant effect of heat stress; (3) bcl 2 and c myc transcription of both significant correlation. The above results suggest that VIP and EGF and other lung regulatory peptides can enhance the anti-oxidative capacity of bronchial epithelial cells by up-regulating bcl 2 gene expression; c myc gene encoding product may be upstream regulator of bcl 2 gene transcription.