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[目的]观察苯吸入染毒对小鼠的周围血象、尿中苯巯基尿酸(SPMA)和8-羟基脱氧鸟苷(8-OHd G)的影响,探讨苯暴露对血液系统和DNA氧化损伤的作用。[方法]将120只昆明种雄性小鼠随机分为4组,每组30只,低、中、高组苯染毒质量浓度分别为375、750和1 500 mg/m3,对照组吸入空气。采用静式吸入染毒法,每天2 h,每周5 d,持续染毒3个月。分别于染毒后1、2、3个月时,每组随机处死10只小鼠,检测周围血象、尿中SPMA和8-OHd G的水平。[结果]白细胞、红细胞、淋巴细胞计数均随着染毒浓度的升高而降低(P<0.05);尿中SPMA及8-OHd G水平随着苯染毒浓度的增加而升高(P<0.05)。高浓度组染毒3个月时尿中8-OHd G水平明显高于1、2个月(P<0.05)。尿中SPMA及8-OHd G与白细胞计数均呈负相关(rSPMA=-0.718,r8-OHd G=-0.971,均P<0.01)。[结论]亚慢性苯暴露可导致小鼠血液系统和DNA氧化损伤。
[Objective] To observe the effects of benzene inhalation exposure on the peripheral blood of mice, the concentrations of phenylmercapturic acid (SPMA) and 8-OHdG (8-OHdG) in mice, and the effects of benzene exposure on blood system and DNA oxidative damage effect. [Method] 120 male Kunming mice were randomly divided into 4 groups with 30 rats in each group. The concentrations of benzene in low, medium and high groups were 375, 750 and 1500 mg / m3, respectively. The control group inhaled air. The use of static inhalation exposure method, 2 h daily, 5 d per week, continued exposure to poison for 3 months. At 1, 2, and 3 months after the treatment, 10 mice were randomly sacrificed in each group to detect the levels of peripheral blood, urine SPMA and 8-OHdG. [Results] The counts of white blood cells, erythrocytes and lymphocytes decreased with the increase of exposure concentration (P <0.05). The levels of SPMA and 8-OHd G in urine increased with the increase of benzene exposure (P < 0.05). Urine levels of 8-OHd G in high concentration group were significantly higher than those in 1 and 2 months (P <0.05) at 3 months. Urinary SPMA and 8-OHd G were negatively correlated with leukocyte counts (rSPMA = -0.718, r8-OHd G = -0.971, all P <0.01). [Conclusion] Subchronic benzene exposure can lead to the blood system and DNA oxidative damage in mice.