ISOLATION AND IDENTIFICATION OF THE RELATED GENE MEDIATING γ-RADIATION-INDUCED APOPTOSIS IN HUMAN HL

• 出 处: 西安交通大学学报(英文版)
• 发表日期: 1999年2期
• 关键词: ISOLATION AND IDENTIFICATION RNA

TO IDENTIFY THE MEMBER OF THE CASPASE FAMILY PROTEASES INVOLVED IN γ RADIATION INDUCED APOPTOSIS IN HL 60 CELLS, USING DEGENERATED OLIGONUCLEOTIDE PRIMERS ENCODING THE HIGHLY CONSERVED PEPTIDES, WHICH WERE PRESENT IN ALL KNOWN CASPASES, RT PCR WAS PERFORMED ON POLY (A) RNA FROM THE γ RADIATION INDUCED APOPTOTIC HL 60 CELLS. THEN, CLONED AND SEQUENCED TO IDENTIFY THE AMPLIFIED DNA FRAGMENTS. THE RESULTS SHOWED THAT THE AMPLIFIED DNA FRAGMENTS WERE IDENTIFIED WITH A PART OF CASPASE 3 CDNA. IT INDICATE...“,”To identify the member of the caspase family proteases involved in γ radiation induced apoptosis in HL 60 cells, using degenerated oligonucleotide primers encoding the highly conserved peptides, which were present in all known caspases, RT PCR was performed on poly (A) RNA from the γ radiation induced apoptotic HL 60 cells. Then, cloned and sequenced to identify the amplified DNA fragments. The results showed that the amplified DNA fragments were identified with a part of caspase 3 cDNA. It indicated that caspase 3 was involved in γ radiation induced apoptosis in HL 60 cells and may be the pivotal element of radiation induced apoptosis.