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本工作以罗汉果叶为材料,在 MS 附加 Ippm6-BA 和0.5ppm IBA 的培养基上,研究了叶外植体植株再生及其组织分化的过程。叶外植体在培养基上培养一周后,叶肉细胞已明显增大,在外植体切口端附近,叶肉细胞分裂并形成具浓厚细胞质、无叶绿体的分生细胞团。稍后,它们增殖并形成为叶状体,约在培养三周后,从叶状体的近表层部份的细胞又重新形成新的分生细胞团,并进一步发展为不定芽。为了使小植株生根,待小苗伸长至2厘米以后,即可切下转入 MS 加 NAA(0.25-0.5ppm)的培养基上,大约10天左右,即可从小苗基部的切口附近产生出健壮的根系。
In this work, we used the Lo Han Guo leaf as the material, and studied the regeneration of plant explants and their differentiation process on the medium of MS supplemented with Ippm6-BA and 0.5ppm IBA. Leaf explants cultured in culture medium for one week, the mesophyll cells have been significantly increased, near the explant incision end, mesophyll cell division and the formation of a thick cytoplasm, chloroplast meristematic cell mass. Later, they proliferated and formed fronds, and about three weeks after the culture, new meristematic cells were newly formed from cells in the near-surface portion of the frond and further developed into adventitious buds. In order to root the plantlets, the seedlings can be cut into medium with MS plus NAA (0.25-0.5ppm) after they are elongated to 2cm. Approximately 10 days, they can be cut out from the incision of the basal part of the seedlings Sturdy roots.