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目的:建立脑得生软胶囊中葛根素和羟基红花黄色素A的含量测定HPLC方法。方法:色谱柱Hypersil C_(18)(250 mm×4.6 mm,5μm),流动相甲醇-乙腈-0.2%磷酸溶液(16:3:81),检测波长250 nm(测葛根素)、403 nm(测羟基红花黄色素A),流速1.2 ml·min~(-1)。结果:葛根素和羟基红花黄色素A的线性范围分别为32～162μg·ml~(-1)(r=0.999 9)和7.3～116.8μg·ml~(-1)(r=0.999 9),其回收率分别为101.3%(RSD=1.1%)和99.8%(RSD=1.3%,n=6)。结论:方法简便、灵敏、重复性好,可作为脑得生软胶囊的质量控制方法。
Objective: To establish HPLC method for determination of puerarin and hydroxysafflor yellow A in Naodesheng Soft Capsule. Methods: The Hypersil C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was methanol-acetonitrile-0.2% phosphoric acid solution (16:3:81). The detection wavelength was 250 nm (measured puerarin) and 403 nm. Hydroxysafflor yellow A) was measured at a flow rate of 1.2 ml·min -1 . Results: The linear ranges of puerarin and hydroxysafflor yellow A were 32-162 μg·ml -1 (r=0.999 9) and 7.3～116.8 μg·ml -1, respectively (r=0.999 9). The recovery rates were 101.3% (RSD=1.1%) and 99.8% (RSD=1.3%, n=6), respectively. Conclusion: The method is simple, sensitive and reproducible. It can be used as a quality control method for Naodesheng soft capsule.