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使用质粒DNA评价法(Plasmid DNA assay)研究了在北京市市区、郊区卫星城市南口镇和清洁对照点十三陵水库地区采集的PM10和PM2.5样品及部分源颗粒物的生物活性.结果表明,沙尘暴期间采集的颗粒物对DNA的生物活性小于非沙尘暴期间采集的样品.不同时间和地点采集的大气颗粒物样品对质粒DNA的损伤差别很大,它们的TM50(造成50％的DNA损伤所需的剂量)相差可高达一个数量级,其原因,可能是北京市大气颗粒物中矿物质量百分比的变化引起了生物活性较强的细颗粒物相对含量的改变.同PM10相比,大部分PM2.5样品的生物活性相对较大.最后,对比水溶和总体颗粒物质粒DNA评价的结果得出,大气颗粒物中的水溶组分是其生物活性的主要来源.
The bioactivity of PM10 and PM2.5 samples and some of the particulate matter collected from the urban area of Shijiazhuang, a suburb satellite city in Nankou Town and the clean control point Shisanling Reservoir were studied by using Plasmid DNA assay.The results showed that , The particulate matter collected during the sandstorm had less biological activity to DNA than the sample collected during the non-sandstorm.The atmospheric particulate matter samples taken at different times and locations had very different damage to the plasmid DNA, and their TM50 (required to cause 50% DNA damage Dose) can be as much as one order of magnitude, probably due to changes in the percentage of minerals in the airborne particulates in Beijing causing changes in the relative abundance of more bioactive fine particles than in PM 10. Most PM2.5 organisms Finally, comparing the results of DNA evaluation of water-soluble and total particulate matter particles, it is concluded that the water-soluble component of atmospheric particulate matter is the main source of biological activity.