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以象草幼穗为外植体材料、改良的MS为基本培养基,研究了不同外源激素组合对不同发育时期幼穗愈伤组织诱导和植株再生能力的影响。结果表明,象草幼穗离体培养最适长度为2~5 cm;愈伤组织诱导培养基中添加4.0 mg/L 2,4-D+0.05 mg/L KT和4.0 mg/L 2,4-D+0.10 mg/L KT时,颗粒状愈伤组织诱导率分别为79.0%和72.6%;转入添加3.0 mg/L 2,4-D+0.2 mg/L 6-BA的继代培养基,分别有40.9%和74.0%的愈伤组织保持颗粒状结构;在添加2.0 mg/L CPPU+0.01 mg/L NAA和0.50 mg/L KT+0.5 mg/L IAA的分化培养基上,经过继代培养后的颗粒状愈伤组织的成苗率分别为36.4%和38.5%,总成苗率达50.2%和43.9%。3片叶大小的幼苗转入附加NAA 0.50 mg/L的1/2 MS壮根培养基,试管苗移栽成活率达95%以上。在继代培养过程中逐代选择外表呈白色、干燥、紧密、颗粒状的愈伤组织,是提高其植株再生能力的有效方法。
Using young grass as explants and modified MS as basic medium, we studied the effects of different exogenous hormones combinations on callus induction and plant regeneration ability of young panicles at different developmental stages. The results showed that the optimum length of in vitro culture of G. gracilis was 2 ~ 5 cm. In the callus induction medium, 4.0 mg / L 2,4-D + 0.05 mg / L KT and 4.0 mg / L 2,4 -D + 0.10 mg / L KT, the induction rates of granular callus were 79.0% and 72.6%, respectively. When the medium supplemented with 3.0 mg / L 2,4-D and 0.2 mg / L 6-BA With 40.9% and 74.0% callus respectively. On the differentiation medium supplemented with 2.0 mg / L CPPU + 0.01 mg / L NAA and 0.50 mg / L KT + 0.5 mg / L IAA, After subculture, the seedling rates of granular callus were 36.4% and 38.5% respectively, and the total seedling rate was 50.2% and 43.9%. Three-leaf-sized seedlings were transferred to ½ MS rooted medium supplemented with 0.50 mg / L NAA, and the survival rate of transplanted test-tube seedlings was over 95%. In the process of subculture selection, the appearance of white, dry, dense, granular callus, is an effective way to improve its plant regeneration ability.