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采用振荡培养(移除根尖边缘细胞)和静置培养(保持边缘细胞附着在根尖)方法,对比研究盐胁迫对黑豆根系生长和根尖边缘细胞发育、根系Na+、K+含量的影响以及根系生理特性的变化。结果显示:100和200 mmol.L-1NaCl处理抑制边缘细胞发育,引起根系相对电导率和MDA含量增加。振荡培养去除根尖边缘细胞处理36 h,黑豆根相对伸长率、根尖K+含量明显低于对应NaCl浓度的静置培养处理,同时根尖Na+含量、相对电导率和MDA含量在去除边缘细胞后显著增加。说明包裹于根尖的边缘细胞通过调节Na+和K+的吸收和维持较高的细胞膜完整性,以适应盐害环境。
The effects of salt stress on root growth and root border cells development and root Na + and K + contents in black bean were studied by shaking culture (removal of apical border cells) and stationary culture (keeping edge cells attached to apical root tips) Changes in physiological characteristics. The results showed that: 100 and 200 mmol.L-1NaCl inhibited the development of marginal cells, resulting in relative root conductivity and MDA content increased. After sham culture for 36 h, the relative elongation and the content of K + in root tips were significantly lower than those in NaCl solution at 36 h after root-tip removal. At the same time, the content of apical Na +, the relative conductivity and the content of MDA in removing marginal cells After a significant increase. The results showed that the cells wrapped in the apical border cells could adapt to the salt damage environment by regulating the absorption of Na + and K + and maintaining the integrity of the cell membrane.