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采用生化和细胞生物学方法研究了不同剂量甲基汞对细胞膜系统损伤作用及机理。结果红细胞膜、脑肝肾微粒体膜Ｔ－ＡＴＰ酶、Ｍｇ￣（＋＋）－ＡＴＰ酶、Ｎａ￣＋－Ｋ￣＋－ＡＴＰ酶活性与对照组相比显著降低，并随剂量增加而下降。红细胞膜Ｎａ￣＋－Ｋ￣＋－ＡＴＰ酶与脑肾微粒体膜Ｎａ￣＋－Ｋ￣＋－ＡＴＰ酶活性呈正相关。红细胞膜、脑微粒体膜ＳＨ基含量均显著降低（Ｐ＜０．０１）。肾ＳＨ基含量与肾三种ＡＴＰ酶均呈负相关，脑ＳＨ基含量与脑ＡＴＰ酶呈正相关。红细胞膜、脑肝肾微粒体膜荧光偏振度增高，膜流动性下降，血浆中ＬＤＨ活性增高而细胞内ＬＤＨ活性则下降，通透性增强。脑肝的线粒体呼吸酶活性与对照组相比呈下降（Ｐ＜０．０５）。肝脾肾脑细胞￣３Ｈ－ＴｄＲ掺入量（ｃｐｍ值）受影响，ＤＮＡ合成和ＵＤＳ修复能力，在高剂量组受抑制，低剂量组则有刺激作用。骨髓细胞ＳＣＥ频率，随着剂量增加ＳＣＥ频率也随之增高（ｐ＜０．００１）。细胞周期进程，从Ｇ＿０／Ｇ＿１期进入Ｓ期的脾细胞数增多，Ｓ期进入Ｇ＿２／Ｍ期则脾细胞数显著减少、ＤＮＡ合成已受到抑制。
Biochemical and cell biology methods were used to study the effects and mechanisms of different doses of methyl mercury on the cell membrane system. Results The activity of Na ~ + -K ~ + -ATPase in erythrocyte membrane, brain-stem-kidney microsomal membrane T-ATPase, Mg ~ (++) - ATPase and Na ~ + -K ~ + -ATPase decreased significantly with the increase of dose. The Na ~ + -K ~ + -ATPase of erythrocyte membrane was positively correlated with Na ~ + -K ~ + -ATPase activity of cerebrospinal fluid microsome membrane. The contents of SH in erythrocyte membrane and brain microsomal membrane were significantly decreased (P <0.01). The content of SH in kidneys was negatively correlated with the three ATPases in kidneys, while the content of SH in brains was positively correlated with brain ATPase. Erythrocyte membrane, brain, liver and kidney microsomal membrane fluorescence polarization increased membrane fluidity decreased, plasma LDH activity increased while intracellular LDH activity decreased, permeability increased. Mitochondrial respiration enzyme activity in the brain decreased compared with the control group (P <0.05). 3h-TdR incorporation (cpm values) of hepatocytes, spleen and kidney cells were affected. The ability of DNA synthesis and UDS repair was inhibited in high dose group and stimulated in low dose group. SCE frequency of bone marrow cells, with increasing doses of SCE frequency also increased (p <0.001). During the cell cycle progression, the number of splenocytes entering G phase from G_0 / G_1 phase increased, while the G_2 / M phase phase S decreased the number of splenocytes and the DNA synthesis was inhibited.