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采用SD雄性大鼠16只,随机分为对照组和羟基脲组,后者喂羟基脲3次/周,前者喂生理盐水.处死前第10天和第2天给其皮下注射四环素,以便有骨表面形成双层荧光,表明分两次注射四环素期间骨形成的情况.3个月后取两组大鼠胫骨中段进行切片,用甲基丙烯酸甲脂行不脱钙骨包埋,被制成20μ的薄骨片用图像分析仪、电脑和形态程度体视学等测算和分析.羟基脲组与对照组比较:动态参数中内骨膜面皮质骨的骨吸收周长百分数增加156%(P<0.05),指示破骨细胞所致的骨吸收明显增加:同时,机体为了阻止皮质骨的大量吸收,促使成骨细胞的数量、功能也增加,表现为荧光标记周长百分数增加80%(P<0.05)、骨矿化沉积率增加397%(P<0.05)和骨形成率增加529%(P<0.05),出现骨形成加速和骨吸收增加这样一种皮质骨高转化率的局面.但是,从荧光周长与吸收周长之比来看,羟基脲组实际上减少36%,说明了骨吸收大于骨形成且最终在静态参数中呈现出皮质骨面积百分数减少7%(P<0.001)、骨髓腔面积百分数增加28%(P<0.01),
Sixteen SD male rats were randomly divided into control group and hydroxyurea group, the latter fed with hydroxyurea 3 times / week, the former fed with saline, and on the 10th and 2nd day before sacrifice, they were subcutaneously injected with tetracycline so as to have Double-layer fluorescence on the surface of the bone indicates bone formation during two injections of tetracycline.Three months later, the middle part of the tibia was dissected from two groups of rats and embedded with non-decalcified bone with methylmethacrylate 20μ thin bone slices were measured and analyzed by using image analyzer, computer and stereological degree of stereology.Hydroxyurea group compared with the control group: the percentage of periarterial cortical bone resorption perimeter in the dynamic parameters increased by 156% (P < 0.05), indicating a significant increase in bone resorption induced by osteoclasts: At the same time, the body in order to prevent the massive absorption of cortical bone, promote the number and function of osteoblasts, showing an increase of 80% in the percentage of fluorescent markers perimeter (P < 0.05), bone mineralization deposition rate increased 397% (P <0.05) and bone formation rate increased 529% (P <0.05), there is a bone formation accelerated and bone resorption increased such a high conversion rate of cortical bone However, From the ratio of fluorescence perimeter to absorption perimeter, the hydroxyurea group actually decreased by 36%, said Bone resorption was clearly greater than bone formation and eventually showed a 7% (P <0.001) decrease in cortical bone area percentage in static parameters, a 28% increase in bone marrow cavity area (P <0.01)