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目的检测系统性红斑狼疮(SLE)患者CD4+CD25+调节性T细胞(Treg)上Ⅰ型干扰素受体(IFNAR)的分布格局,了解Ⅰ型干扰素对SLE患者CD4+CD25+Treg产生直接影响的作用靶点。方法选取2010年9月-2011年10月间20例初次确诊的SLE患者(SLE组)和20例健康女性(对照组),分离SLE患者和对照组的外周血单个核细胞,采用流式细胞术测定CD4+CD25+Treg上IFNAR的表达。结果①IFNAR1、IFNAR2在Treg和CD4+CD25 T细胞表面均有表达;两组Treg表面IFNAR1和IFNAR2的表达水平均高于CD4+CD25 T细胞。②与对照组相比,SLE组Treg表面IFNAR1表达的平均荧光强度明显增高(P=0.001)。③SLE组Treg表面IFNAR1表达平均荧光强度与疾病活动指数评分呈正相关(rs=0.505,P=0.023)。结论 SLE患者CD4+CD25+Treg表面相对高表达IFNAR1且与疾病活动性相关,提示Ⅰ型干扰素以Treg上IFNAR为靶点在SLE发病机制中可能发挥重要作用,为SLE等自身免疫性疾病治疗寻找新的干预手段提供了理论基础。
Objective To detect the distribution of type Ⅰ interferon receptor (IFNAR) on CD4 + CD25 + regulatory T cells (Treg) in patients with systemic lupus erythematosus (SLE) and to find out the direct effect of type Ⅰ interferon on CD4 + CD25 + Treg in SLE patients The role of target. Methods From September 2010 to October 2011, 20 newly diagnosed patients with SLE (SLE group) and 20 healthy women (control group) were enrolled. Peripheral blood mononuclear cells (PBMCs) from patients with SLE and controls were isolated and treated with flow cytometry The expression of IFNAR on CD4 + CD25 + Treg was measured. Results ①IFNAR1 and IFNAR2 were expressed on the surface of both Treg and CD4 + CD25 T cells. The levels of IFNAR1 and IFNAR2 on Treg surface were higher than those on CD4 + CD25 T cells. ② Compared with the control group, the average fluorescence intensity of IFNAR1 expression on Tregs in SLE group was significantly increased (P = 0.001). ③ The average fluorescence intensity of IFNAR1 expression on Tregs in SLE group was positively correlated with disease activity index (rs = 0.505, P = 0.023). Conclusion The expression of IFNAR1 is relatively high on the surface of CD4 + CD25 + Treg in patients with SLE, indicating that interferon type Ⅰ may play an important role in the pathogenesis of SLE with the target of IFNAR on Treg, which is the treatment of autoimmune diseases such as SLE Find a new means of intervention provides the theoretical basis.