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目的研究CD44v6和c-erbB-2癌基因蛋白在胰腺癌组织中的表达与组织学分级和转移的关系,并探讨CD44v6和c-erbB-2癌基因蛋白的相关性。方法应用免疫组织化学方法检测胰腺癌组织以及癌旁组织CD44v6和c-erbB-2癌基因蛋白表达。结果 CD44v6和c-erbB-2癌基因蛋白阳性率在胰腺癌组分别为69.0%及52.4%,均显著高于其在癌旁组的21.2%及8.1%(P<0.01)。胰腺癌中,CD44v6阳性率在组织分级Ⅰ～Ⅱ级组为50.0%,显著低于Ⅲ级组的86.4%(P<0.05),其在有淋巴结转移组阳性率为92.3%,显著高于无淋巴转移组的58.6%(P<0.05);c-erbB-2癌基因蛋白阳性率在组织分级Ⅰ～Ⅱ级组为35.0%,显著低于组织分级Ⅲ级组的68.2%(P<0.05),其在有淋巴结转移组阳性率为76.9%,显著高于在无淋巴转移组的41.3%(P<0.05);两者在胰腺癌中的表达具有相关性(γ=0.393,P<0.05)。结论 CD44v6和c-erbB-2癌基因蛋白均与胰腺癌的分化、转移有关;两者的高表达提示肿瘤恶性程度高,易发生淋巴结转移。
Objective To investigate the relationship between the expression of CD44v6 and c-erbB-2 oncogene proteins in pancreatic cancer and histological grade and metastasis, and to explore the relationship between CD44v6 and c-erbB-2 oncogene proteins. Methods The expressions of CD44v6 and c-erbB-2 oncogene proteins in pancreatic cancer tissues and adjacent tissues were detected by immunohistochemistry. Results The positive rates of CD44v6 and c-erbB-2 oncogene protein were 69.0% and 52.4% in pancreatic cancer group, respectively, which were significantly higher than those in 21.2% and 8.1% (P <0.01) of paracancer group. In pancreatic cancer, the positive rate of CD44v6 was 50.0% in grade Ⅰ ~ Ⅱ group and 86.4% in grade Ⅲ group (P <0.05). The positive rate of CD44v6 was 92.3% in group with lymph node metastasis, which was significantly higher than that in group without lymph node metastasis The positive rate of c-erbB-2 oncogene protein was 35.0% in the tissue gradeⅠ-Ⅱ group and 68.2% (P0.05) in the lymph node metastasis group (58.6%, P <0.05) , The positive rate was 76.9% in lymph node metastasis group, which was significantly higher than that in non-lymphatic metastasis group (41.3%, P <0.05). There was a correlation between the two expressions in pancreatic cancer (γ = 0.393, P <0.05) . Conclusions Both oncogene protein of CD44v6 and c-erbB-2 are related to the differentiation and metastasis of pancreatic cancer. The high expression of CD44v6 and c-erbB-2 suggests that the tumor is highly malignant and prone to lymph node metastasis.