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目的 探讨将胃癌患者术前、术后血清 IL - 12水平作为机体自主免疫功能状态的监测指标的可行性 .方法 收集本院 2 0 0 0 - 10 / 2 0 0 1- 12确诊为胃癌的连续住院病例 72例 ,按术后静脉支持治疗方案不同分为两组 ,分别于术前 1d、术后8d采血 .另外取同期消化性溃疡住院患者血清作为对照 .采用双抗体夹心 EL ISA法测定 IL- 12水平 ,用 DKT系列 m Ab间接荧光法由流式细胞仪测定 T淋巴细胞亚群 .结果 胃癌患者术后 8d CD4 + T淋巴细胞亚群测定 ,应用全胃肠外营养(TPN)组为 36 .81± 5 .37,较术前值 32 .11± 6 .5 2明显升高(P<0 .0 5 ) .术前血清 IL - 12水平两组分别为 (44 .8± 2 1.3)pg· m L- 1和 (43.2± 17.9) pg· m L- 1 ,较正常对照组 (10 9.5± 6 2 .9) pg· m L- 1 明显降低 (P<0 .0 1) ,术后 8d两组血清IL - 12水平不同程度升高 ,应用 TPN组为 (73.4± 18.6 ) pg·m L- 1 较术前明显升高 (P<0 .0 1) ,较非 TPN组水平 (5 2 .4±2 2 .4 ) pg· m L- 1明显升高 (P<0 .0 5 ) .结论 胃癌患者血清IL - 12水平较正常人低 ,术后随着机体免疫功能的恢复 ,血清IL - 12水平有所上升 .应用 TPN支持治疗有利于术后机体免疫功能恢复 ,IL- 12水平上升更明显 .血清 IL- 12水平可以作为胃癌术后机体免疫功能
Objective To explore the feasibility of using preoperative and postoperative serum levels of IL - 12 as a monitoring indicator of autoimmune function in patients with gastric cancer.Methods To collect consecutive cases of gastric cancer diagnosed in our hospital from 2000/10 to 2000-12 72 cases of hospitalized patients were divided into two groups according to the different postoperative venous supportive treatment programs, blood samples were collected on the first day before operation and on the eighth day after operation.Serum of peptic ulcer inpatients was taken as control.Serum IL- - 12, and T lymphocyte subsets were determined by flow cytometry with the indirect fluorescent method of DKT series m Ab.Results The CD4 + T lymphocyte subsets of gastric cancer patients were measured 8 days after operation and the total parenteral nutrition (TPN) group was 36.81 ± 5.37, which was significantly higher than the preoperative value of 32.11 ± 6.52 (P <0.05) .The level of IL - 12 in preoperative serum was (44.8 ± 2.1) ) pg · m L -1 and (43.2 ± 17.9) pg · m L -1, respectively, were significantly lower than those in the normal control group (10 9.5 ± 6.22) pg · m L -1 (P <0.01) Serum levels of IL - 12 in the two groups increased to different extents at 8th day after operation. The levels of (73.4 ± 18.6) pg · m L -1 in TPN group were significantly higher than those before operation (P <0.01) (5 (P <0.05) .Conclusion The serum level of IL - 12 in patients with gastric cancer is lower than that in normal people. With the recovery of immune function, Serum IL - 12 levels increased.Application of TPN supportive treatment is conducive to postoperative recovery of immune function, IL - 12 levels increased more significantly.Serum IL - 12 levels can be used as postoperative gastric cancer immune function