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目的研究脑缺血时干扰素γ与细胞间黏附分子(ICAM)1的表达是否具有相关性。方法将雄性SD大鼠54只随机分成6组(A、B、C、D、E、F组),每组9只。再将每组大鼠随机分成实验组(6只)和对照组(3只)。A、B、C、D、E、F实验组用埋线法制造大鼠局灶性脑缺血再灌注模型,分别于再灌注后1、3、6、9、12、24h处死大鼠,取大鼠脑组织,应用免疫组织化学方法分别检测干扰素γ及ICAM1的表达。高倍显微镜下计数10个不同视野阳性细胞的平均值。结果大鼠脑缺血再灌注1、3、6、9、12、24h时,干扰素γ及ICAM1的表达逐渐增高,其中干扰素γ阳性的细胞是圆形、以细胞核为主的淋巴细胞,1h阳性细胞表达数为3.2±1.3;6h为7.0±2.2;24h为14.8±1.8。ICAM1阳性细胞是扁平的血管内皮细胞,1h的表达数为12.1±2.1;6h为19.3±3.2;24h为27.6±2.8。结论脑缺血时干扰素γ、ICAM1的表达随时间延长动态地增高,两者具有相关性。干扰素γ也可能是脑缺血时ICAM1表达上调的前炎性细胞因子。
Objective To investigate whether there is a correlation between the expression of intercellular adhesion molecule (ICAM) 1 and interferon gamma in cerebral ischemia. Methods 54 male Sprague-Dawley rats were randomly divided into 6 groups (A, B, C, D, E and F), with 9 rats in each group. Each group of rats were randomly divided into experimental group (6) and control group (3). Rats in group A, B, C, D, E, F were subjected to focal cerebral ischemia / reperfusion with embedding method. Rats were sacrificed at 1,3,6,9,12,24 h after reperfusion. The brain tissues of rats were taken out and the expressions of interferon gamma and ICAM1 were detected by immunohistochemistry. High magnification microscope counting the average of 10 different fields of vision positive cells. Results At 1, 3, 6, 9, 12 and 24 h after cerebral ischemia, the expression of interferon γ and ICAM1 gradually increased. Among them, the positive cells of interferon γ were round and the nucleus dominated lymphocytes, The number of positive cells at 1 h was 3.2 ± 1.3; at 6 h 7.0 ± 2.2; at 24h 14.8 ± 1.8. ICAM1 positive cells were flat vascular endothelial cells, the expression of 1h was 12.1 ± 2.1; 6h 19.3 ± 3.2; 24h was 27.6 ± 2.8. Conclusions The expression of interferon gamma and ICAM1 in cerebral ischemia increases dynamically with time, both of them are correlated. Interferon gamma may also be a proinflammatory cytokine up-regulated in ICAM1 expression during cerebral ischemia.