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通过羊水分析所获得的情报必须能直接反映胎儿的情况。为此,作者探讨了羊水成份、母儿血清、包围羊膜腔的组织、羊膜腔内与羊水接触的胎儿各脏器等的LDH同功酶谱的变动,并观察了羊水中LDH的来源及随着胎儿发育的变化以及流产、异常分娩时羊水LDH同功酶谱的改变。作者将新鲜血清、不混浊的羊水及胎儿脏器匀浆的上清液经聚丙酰胺薄层电泳后,用硝基四氮唑蓝、甲硫酚嗪、40%乳酸钠染色,干燥后进行光密度测定。同功酶谱的5个区带,从阳极起按LDH至LDH_5表示,并比较其在各妊娠时期的活性比率。结果如下: 1.母血清:以LDH_(1、2)的嗜氧型占优势,在妊
The information obtained by amniotic fluid analysis must directly reflect the condition of the fetus. To this end, the authors discussed the amniotic fluid composition, mother’s serum, the surrounding amniotic tissue, fetal amniotic fluid in contact with amniotic fluid and other organs of the fetal LDH changes in the zymogram, and observed the source of amniotic fluid and LDH Changes in fetal development and miscarriage, abnormal delivery of amniotic fluid LDH zymogram changes. After the supernatant of fresh serum, turbid amniotic fluid and fetal organ homogenate was electrophoresed on thin layer of polyacrylamide, it was stained with nitro-tetrazolium blue, methothiazine and 40% sodium lactate and dried to measure the optical density Determination. The five bands of the zymogram were expressed as LDH to LDH_5 from the anode and their activity ratio was compared during each gestation period. The results are as follows: 1. Maternal serum: LDH_ (1, 2) predominance of oxygenation in pregnancy