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目的:测定贵州不同产地的红土茯苓中指标性成分薯蓣皂苷元。方法:采用RP-HPLC法测定红土茯苓中薯蓣皂苷元的含量,色谱条件为依利特Hypersil ODS2色谱柱(5μm,4.6×250mm),流动相甲醇—0.2%磷酸(76∶24),流速1.0ml/min;检测波长203nm,柱温30℃,薯蓣皂苷元理论塔板数不得低于5000。结果:在此色谱条件下,薯蓣皂苷元在0.010525～2.105μg范围内与峰面积呈良好线性关系;平均加样回收率(n=9)为100.4%(RSD=1.85%),贵州不同产地的红土茯苓中薯蓣皂苷元的含量在0.0024%～0.0035%之间。结论:RP-HPLC法简单易行,专属性强,准确度高,重复性好,可作为红土茯苓药材内在质量控制方法。
Objective: To determine the content of diosgenin in red tuckahoe in different areas of Guizhou Province. Method: The content of diosgenin in Rhodiola sibirica was determined by RP-HPLC. The chromatographic conditions were Elite Hypersil ODS2 column (5μm, 4.6 × 250mm), mobile phase methanol-0.2% phosphoric acid (76:24) / min; detection wavelength of 203nm, column temperature 30 ℃, diosgenin theoretical plates no less than 5000. Results: Under the chromatographic conditions, diosgenin showed a good linear relationship with the peak area in the range of 0.010525 ~ 2.105μg. The average recovery was 100.4% (RSD = 1.85%), The content of diosgenin in laterite Poria is between 0.0024% and 0.0035%. Conclusion: RP-HPLC method is simple, specific, accurate, reproducible, and can be used as the intrinsic quality control method of Smilax china.