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目的 探讨非小细胞肺癌 (NSCLC)患者外周血微转移的分子诊断的临床意义。方法 应用逆转录聚合酶链反应 (RT -PCR)技术 ,对 31例肺癌患者和 10例肺良性病变患者外周血中MUC1基因mRNA表达进行检测。结果 术前 10例NSCLC患者检测到外周血肺癌微转移 ,外周血微转移与肺癌组织学类型 ,细胞分化程度及P -TNM分期均存在密切关系 (P <0 .0 5 )。 10例肺良性病变患者的外周血中未检测到MUC1mRNA表达。结论 MUC1基因mRNART -PCR法检测肺癌患者外周血微转移具有较高的特异性和敏感性 ,可为制定治疗方案和评估预后提供重要参考依据。
Objective To investigate the clinical significance of molecular diagnosis of peripheral blood micrometastases in patients with non-small cell lung cancer (NSCLC). Methods The mRNA expression of MUC1 in peripheral blood of 31 patients with lung cancer and 10 patients with benign lung disease was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results Micrometastases of peripheral lung cancer were detected in 10 patients with preoperative NSCLC. There was a close relationship between histological type, cell differentiation and P-TNM stage in peripheral blood micrometastases (P <0.05). MUC1 mRNA expression was not detected in the peripheral blood of 10 patients with benign lung disease. Conclusion The detection of MUC1 gene mRNART-PCR in detecting peripheral blood micrometastasis in patients with lung cancer has high specificity and sensitivity, which may provide an important reference for the development of treatment options and prognosis.