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目的探讨青蒿素联合~(60)Coγ射线照射后对人宫颈癌HeLa和SiHa细胞的DNA损伤。方法采用甲基四唑蓝(MTT)法检测不同浓度青蒿素对人宫颈癌HeLa和SiHa细胞的抑制效应,确定青蒿素的最适实验浓度;采用单细胞凝胶电泳技术(SCGE)检测照射后HeLa和SiHa细胞DNA的损伤情况。结果青蒿素对HeLa和SiHa细胞的抑制率存在剂量-效应关系;SCGE结果显示:两种细胞的单药组与对照组的彗星细胞检测指标(拖尾率、尾长、OLIVE尾矩和彗尾DNA%)无明显差异(P>0.05);在相同照射剂量下,青蒿素加照射组的HeLa细胞的彗星细胞检测指标高于单纯照射组(P<0.05),而SiHa细胞差异无统计学意义(P>0.05)。结论青蒿素本身不能引起He-La和SiHa细胞DNA损伤,但辐照后可致HeLa细胞DNA损伤加重,增加了HeLa细胞的辐射敏感性,而对SiHa细胞没有辐射增敏作用。
Objective To investigate the DNA damage of human cervical carcinoma HeLa and SiHa cells induced by artemisinin combined with ~ (60) Co γ -ray irradiation. Methods Methyltetrazolium (MTT) assay was used to detect the inhibitory effect of different concentrations of artemisinin on human cervical cancer HeLa and SiHa cells. The optimum concentration of artemisinin was determined. Single cell gel electrophoresis (SCGE) DNA damage in HeLa and SiHa cells after irradiation. Results There was a dose-response relationship between artemisinin and HeLa and SiHa cells. The results of SCGE showed that the detection indexes (tailing rate, tail length, OLIVE tail moment and coma) of single cell group and control group (P> 0.05). Under the same irradiation dose, the detection index of comet cells in HeLa cells treated with artemisinin plus irradiation was higher than that in the irradiated groups (P <0.05), but there was no statistical difference in SiHa cells Significance (P> 0.05). Conclusion Artemisinin can not induce DNA damage in He-La and SiHa cells by itself, but irradiation can cause DNA damage in HeLa cells, which increases the radiation sensitivity of HeLa cells and does not sensitize SiHa cells to radiation.