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以3个芋品种‘(石川早生’、‘虾籽芋’、‘叶用芋)球茎茎尖为外植体,进行脱病毒和快繁的结果表明,外植体表面灭菌的最佳方法是剥鳞片→乙醇→新洁尔灭→剥幼叶→氯化汞;适宜茎尖分化的培养基为MS+1.0～2.0mg·L-16-BA+0.2mg·L-1NAA。生物学方法和电镜观察显示:连续3代0.5～0.7mm茎尖剥离培养对芋花叶病毒(DMV)的脱毒率达100%。在培养基MS+0.2mg·L-1NAA中,适量添加6-BA和TDZ,三品种芋的试管苗增殖效果好;附加KT,试管苗生长健壮且利于生根;添加20～100mg·L-1的精胺(Spm),可促进不定芽的发生,与KT配合使用可促使继代增殖和成苗一步完成。完整植株在草炭土:蛭石=1:1的基质中,移栽成活率超过97%,且苗生长健壮。
The results of virus-free and rapid propagation showed that the best method for the surface sterilization of explants was the explants with bulbs of stems and shoots of three taro cultivars ’(Ishikawa early life’, ’shrimp seed taro’, ’leaf taro’) Is flaky scales → ethanol → benzalkonium bromide → stripped young leaves → mercuric chloride; suitable medium for MSCs differentiation is MS + 1.0-2.0 mg · L-16-BA + 0.2 mg · L -1 NAA. The biological methods and electron microscopy showed that the rate of detoxification of Taro mosaic virus (DMV) reached 100% for 3 generations of 0.5-0.7 mm shoot tip peel culture. In the medium of MS + 0.2mg · L-1NAA, 6-BA and TDZ were added in appropriate amount, and the proliferation of three-species plantlets of Taro was good. With the addition of KT, the plantlets grew robustly and were conducive to rooting. Adding 20-100 mg · L -1 Of spermine (Spm), can promote the occurrence of adventitious buds, used in conjunction with KT can promote sub-proliferation and seedling in one step. The intact plant had a transplanted survival rate of more than 97% in a matrix of peat soil: vermiculite = 1: 1 and the seedlings grew robustly.