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本文研究HMBA对人肝癌SMMC-7721细胞的诱导分化作用。细胞生长曲线测定和细胞分裂指数观察显示HMBA可明显抑制细胞增殖,细胞生长抑制率达64.14%,分裂指数抑制率为53.88%。光镜和透射电镜观察可见HMBA能诱导人肝癌SMMC-7721细胞形态和超微结构发生恢复性改变。生化检测或免疫细胞化学方法观察显示,HMBA处理后细胞γ-谷氨酸转肽酶(γ-GT)活性和甲胎蛋白(AFP)、增殖细胞核抗原(PCNA)表达均降低,而酪氨酸-酮戊二酸转氨酶(TAT)活性增强。流式细胞仪分析表明HMBA引起细胞发生G_0/G_1期阻滞。以上结果表明HMBA能有效抑制人肝癌细胞恶性增殖活性,逆转肝癌细胞恶性形态与超微结构特征,改变肝癌细胞相关酶活性和抗原表达,引发G_0/G_1期阻滞,从而对肝癌细胞具有明显的诱导分化作用。
This study was to investigate the effect of HMBA on differentiation of human hepatocellular carcinoma SMMC-7721 cells. Cell growth curve and cell division index observation showed that HMBA could significantly inhibit cell proliferation, cell growth inhibition rate was 64.14%, and mitotic index inhibition rate was 53.88%. Light microscopy and transmission electron microscopy showed that HMBA could induce the restoration of the morphology and ultrastructure of human hepatoma SMMC-7721 cells. Biochemical tests or immunocytochemical methods showed that the activity of γ-glutamate transpeptidase (γ-GT) and the expression of alpha fetoprotein (AFP) and proliferating cell nuclear antigen (PCNA) were decreased after HMBA treatment, while tyrosine was decreased. - Increased ketoglutarate transaminase (TAT) activity. Flow cytometry analysis showed that HMBA induced cell G0/G1 phase arrest. The above results indicate that HMBA can effectively inhibit the malignant proliferation activity of human hepatocellular carcinoma cells, reverse the malignant morphology and ultrastructural characteristics of hepatoma cells, alter the activity of related hepatocellular carcinoma cell enzymes and antigen expression, trigger G0/G1 phase arrest, and thus have obvious effects on hepatoma cells. Induce differentiation.