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目的研究长期慢性缺氧对紫绀型先天性心脏病患儿自体骨髓间充质干细胞(Bone Marrow-derived Mesenchymal Stem Cells,BMSCs)生物学行为的影响。方法通过血气分析紫绀组和非紫绀组骨髓的氧浓度,检测骨髓血BMSCs含量,评估细胞增殖能力,以及BMSCs定向诱导为成骨、成脂及成软骨细胞的能力。给予缺氧缺血清处理后评估BMSCs的抗凋亡能力。结果紫绀组患儿的骨髓氧分压较非紫绀显著降低(P<0.01),两组骨髓的BMSCs的含量无统计学差异,但紫绀组BMSCs的增殖能力,成骨、成脂、成软骨分化能力及抗凋亡能力较非紫绀组显著降低(P<0.05)。结论长期慢性缺氧能明显降低骨髓的氧浓度,并显著减弱BMSCs的增殖、多向分化及抗凋亡能力,但对BMSCs的含量无影响。
Objective To investigate the effects of long-term chronic hypoxia on the biological behavior of Bone Marrow-derived Mesenchymal Stem Cells (BMSCs) in children with cyanotic congenital heart disease. Methods The bone marrow oxygen of cyanotic group and non-cyanotic group was analyzed by blood gas. The content of BMSCs in bone marrow was measured to evaluate the ability of BMSCs to proliferate and to induce osteogenic, adipogenic and chondrogenic cells. The anti-apoptotic ability of BMSCs was assessed after hypoxic-ischemic clearance. Results Compared with non-cyanosis, the partial pressure of oxygen of bone marrow in children with cyanotic group was significantly lower than that of non-cyanosis group (P <0.01). There was no significant difference in the content of BMSCs between the two groups, but the proliferation, osteogenic, adipogenic and cartilage differentiation Ability and anti-apoptotic than non-cyanotic group was significantly lower (P <0.05). Conclusion Long-term chronic hypoxia can significantly reduce the oxygen concentration in bone marrow, and significantly reduce the proliferation, multi-directional differentiation and anti-apoptotic ability of BMSCs, but have no effect on the content of BMSCs.