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目的 探讨己酮可可碱（ｐｅｎｔｏｘｉｆｙｌｌｉｎｅ，ＰＴＸ）对人α１（Ⅰ）前胶原（ＣＯＬ１Ａ１）基因启动子活性的作用及其机制。方法 构建含人ＣＯＬ１Ａ１基因启动于序列－２４８３－＋４２ ｈｐ片段与氯霉素乙酰基转移酶（ＣＡＴ）报告基因的重组体ｐＣＯＬＨ２．５，将其瞬时转染至人皮肤成纤维细胞，加入ＰＴＸ或／和胰岛素样生长因子－１（ＩＧＦ－１）及胰岛素，测定ＣＡＴ活性。结果 ０．４ｍｍｏｌ／Ｌ、２ｍｍｏｌ／Ｌ和１０ｍｍｏｌ／Ｌ的ＰＴＸ使ｐＣＯＬＨ２．５的活性降至对照的（８２±９）％、（５８±８）％与（３２±１３）％。ＩＧＦ－１与胰岛素能促进ｐＣＯＬＨ２．５的活性。ＰＴＸ能拮抗ＩＧＦ－１与胰岛素的这一促进作用。结论 ＰＴＸ能下调人ＣＯＬ１Ａ１启动子的活性，而ＩＧＦ－１与胰岛素能上调该启动子的活性。ＰＴＸ能拮抗ＩＧＦ－１与胰岛素的这一上调作用。
Objective To investigate the effect of pentoxifylline (PTX) on promoter activity of human α1 (Ⅰ) procollagen (COL1A1) gene and its mechanism. Methods Recombinant pCOLH2.5 containing human COL1A1 gene promoter sequence of -2483- + 42 hp and chloramphenicol acetyltransferase (CAT) reporter gene was constructed and transiently transfected into human skin fibroblasts. After addition of PTX or / And insulin-like growth factor-1 (IGF-1) and insulin, CAT activity was measured. Results 0.4mmol / L, 2mmol / L and 10mmol / L of PTX decreased the activity of pCOLH2.5 to (82 ± 9)%, (58 ± 8)% and (32 ± 13)% of the control. IGF-1 and insulin can promote the activity of pCOLH2.5. PTX antagonizes this promoting effect of IGF-1 and insulin. Conclusion PTX can down-regulate the activity of human COL1A1 promoter, while IGF-1 and insulin up-regulate the activity of this promoter. PTX antagonizes this upregulation of IGF-1 and insulin.