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目的:建立HPLC法测定保济口服液浸膏二中厚朴酚、和厚朴酚的含量测定方法。方法:色谱条件:菲罗门C18柱(250mm×4.6mm,5μm)色谱柱,柱温为30℃,流动相为甲醇-水(78∶22),流速为1.0mL/min,检测波长为294nm。结果:厚朴酚、和厚朴酚的回归方程为:厚朴酚Y=1.5264X-1.8032,r2=1.0000,线性范围:4.168~1250.4μg;和厚朴酚Y=1.2620X+0.2406,r2=1.0000,线性范围:2.43~729μg。平均回收率厚朴酚为100.12%,RSD=2.96%(n=6),和厚朴酚为99.01%,RSD=1.85%(n=6)。结论:本法具有样品处理简单、阴性样品无干扰、重现性好、稳定性高等特点,能很好地提高保济口服液浸膏二的质量可控性。
OBJECTIVE: To establish a method for the determination of honokiol and honokiol in Baoji oral liquid extract by HPLC. Methods: The chromatographic conditions were: a Phenomenex C18 column (250 mm × 4.6 mm, 5 μm) with a column temperature of 30 ° C and a mobile phase of methanol-water (78:22) with a flow rate of 1.0 mL / min and a detection wavelength of 294 nm . Results: The regression equations of honokiol and honokiol were as follows: magnolol Y = 1.5264X-1.8032, r2 = 1.0000, linear range: 4.168-1250.4μg; honokiol Y = 1.2620X + 0.2406, r2 = 1.0000, linear range: 2.43 ~ 729μg. The average recoveries of honokiol were 100.12%, RSD = 2.96% (n = 6), honokiol 99.01% and RSD = 1.85% (n = 6). Conclusion: The method has the characteristics of simple sample handling, no interference of negative samples, good reproducibility and high stability, and can well improve the quality controllability of Baoji oral liquid extract II.