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目的 :探讨威草胶囊对尿酸性肾病大鼠的作用机制。方法 :用腺嘌呤加沙丁鱼造模 ,并将大鼠分为正常对照组、模型空白组、小剂量威草胶囊治疗组、大剂量威草胶囊治疗组、别嘌呤醇治疗组。在造模的同时 ,分别给予以上述药物灌胃 ,在实验第 4周 ,活杀所有大鼠 ,取肾组织 ,做肾脏病理组织检查 ,用点计数法计算肾小管的萎缩率 ,炎症细胞浸润面积 ,间质纤维化率 ;应用免疫组织化学技术检测大鼠肾组织TGF β1和TIMP 1(组织基质金属蛋白酶抑制物 -1)的表达情况。结果 :与正常组相比 ,模型空白组和各治疗组TGF β1、TIMP 1阳性表达增高 (P <0 0 1) ;与模型空白组相比 ,治疗组肾小管的萎缩率、炎症细胞浸润面积、间质纤维化率明显减轻 (P <0 0 1) ;TGF β1、TIMP 1阳性表达下降 (P <0 0 1)。结论 :威草胶囊可能通过抑制TGF β1表达 ,抑制细胞外基质和细胞因子的表达分泌 ;通过抑制TIMP 1的表达 ,促进细胞外基质的降解 ,从而减少尿酸性肾病肾间质纤维化。
Objective: To investigate the mechanism of Wei Cao Capsule in rats with uric acid nephropathy. Methods: Adenine plus sardine was used for modeling. The rats were divided into normal control group, model blank group, low dose Weimao capsule treatment group, high dose Weimao capsule treatment group and allopurinol treatment group. At the same time of modeling, the above drugs were respectively given into the gavage. In the 4th week of the experiment, all the rats were killed and killed, the kidneys were removed, the pathological examination of the kidneys was done, the atrophy of renal tubules and the infiltration of inflammatory cells Area and interstitial fibrosis rate. The expression of TGF-β1 and TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) in rat renal tissue were detected by immunohistochemistry. Results: Compared with the normal group, the expression of TGFβ1 and TIMP-1 in the untreated group and the untreated group were significantly increased (P <0.01). Compared with the blank control group, the atrophy rate of renal tubules, infiltration area of inflammatory cells , Interstitial fibrosis rate was significantly reduced (P <0.01); TGF β1, TIMP 1 expression decreased (P <0.01). Conclusion: Weicao Capsule may inhibit renal interstitial fibrosis of uric acid nephropathy by inhibiting the expression of TGF-β1, inhibiting the expression and secretion of extracellular matrix and cytokines, and inhibiting the expression of TIMP-1 and promoting the degradation of extracellular matrix.