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为了探讨外周 NMDA受体是否介导大鼠足底皮下注入蜜蜂毒诱导的持续伤害性行为反应 ,本研究应用动物痛行为学定量方法评价局部用药对持续伤害性行为反应的作用效果。大鼠足底皮下注入蜜蜂毒可以诱导动物产生长达 1h以上的持续、单相性的自发痛反应 ,其表现为自发缩足反射、抬足、舔足甚至咬足行为。注入蜜蜂毒之前局部给予非竞争性的 NMDA受体通道阻断剂氯胺酮和 MK-80 1。局部注入 2 5 m mol/L和 5 0 mmol/L氯胺酮可剂量依赖性地抑制大鼠的缩足次数和抬足、舔足时间 ,其对缩足反射的抑制率分别为 2 0 .90± 2 .88%和 45 .76± 13 .99% ,对抬足、舔足时间的抑制率分别为 3 9.5 3± 10 .0 5 %和 5 9.94±5 .5 3 %。同样 ,局部注入 10 μmol/L和 10 0 μmol/L的 MK-80 1也产生了类似的抑制作用 ,其对缩足反射的抑制率分别为 2 2 .84±3 .12 %和 49.5 3± 5 .3 6% ;对抬足、舔足时间的抑制率分别为 17.49± 5 .67%和 5 3 .49± 3 .87%。然而 ,对侧足底注入氯胺酮和MK-80 1对同侧蜜蜂毒诱导的自发痛反应没有影响 ,说明氯胺酮和 MK-80 1的作用并非全身效应。本实验提示外周 NMDA受体的激活参与蜜蜂毒诱导的持续自发痛反应过程
In order to investigate whether peripheral NMDA receptors mediate the persistent nociceptive response induced by subcutaneous bee venom in the rat’s plantar skin, this study evaluated the effect of topical administration on sustained nociceptive behavior using quantitative animal pain behavior theory. Subcutaneous injection of bee venom in rat plantar can induce sustained and monophasic spontaneous pain reaction in animals for more than 1h, which is characterized by self-contracting reflex, raising foot, licking foot and even biting foot. Topical administration of noncompetitive NMDA receptor channel blockers ketamine and MK-80 1 prior to infusions of the bee venom. Local injection of 25 m mol / L and 50 mmol / L ketamine can inhibit the dose-dependent reduction of the number of rats in foot-lifting and licking-time, and their inhibitory effects on contracting reflex were 20.990 ± 2 .88% and 45 .76 ± 13 .99%, respectively, and the inhibitory rates of raising foot and licking foot were 3 9.53 ± 10. 05% and 59.94 ± 5. 53%, respectively. Similarly, local injection of MK-80 1 at 10 μmol / L and 100 μmol / L produced similar inhibitory effects on the reduction of contracting reflexes of 22.84 ± 3.12% and 49.5 3 ± 5.36%, respectively. The inhibition rates on raising foot and licking feet were 17.49 ± 5 .67% and 53.49% ± 3.87%, respectively. However, contralateral plantar injection of ketamine and MK-80 1 had no effect on ipsilateral spontaneous pain-induced spontaneous pain response, suggesting that the effects of ketamine and MK-80 1 are not systemic. This experiment suggests that activation of peripheral NMDA receptors is involved in a sustained spontaneous pain response induced by bee venom