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本研究使用束缚方法使大鼠接受应激刺激， 然后分别取大鼠应激３ 、６ 、１２、１８ ｈ 和解除束缚后１２ 、２４ 、４８ 、７２ 、９６ ｈ 的淋巴结、脾脏提取物和血清， 与刀豆素Ａ 同时加入正常大鼠肠系膜淋巴结细胞悬液中孵育７２ ｈ 后用噻唑蓝（ＭＴＴ） 比色分析法检测肠系膜淋巴结细胞的转化（ 以光密度ＯＤ 值表示） ， 来观察应激抑制淋巴细胞转化作用的出现和消失过程。结果如下：（１） 应激３ 和６ ｈ 大鼠的淋巴结、脾脏提取物和血清对淋巴细胞的转化都没有明显的影响；（２）应激１２ ｈ， 大鼠的淋巴结、脾脏提取物和血清都出现对淋巴细胞转化的明显抑制作用， 应激１８ｈ 的作用更强；（３） 淋巴结和脾脏提取物对淋巴细胞转化的抑制作用一直持续至束缚解除后９６ ｈ 仍然较强， 但血清对淋巴细胞转化的抑制作用在束缚解除后逐渐减弱， 至９６ ｈ ， 作用基本消失。上述结果说明， 大鼠束缚应激１２ ｈ 以上， 其淋巴结、脾脏和血清同步出现对淋巴细胞转化的抑制作用； 这种抑制作用即使在束缚解除后仍能存在较长时间； 血清的抑制作用时间短于淋巴结和脾脏的抑制作用时间。结果提示， 束缚应激可引起淋巴组织产生一种能抑制免疫功能的应激免疫抑制蛋白（ＩＳＰＳ） ， 并释放到血液中
In this study, rats were subjected to stress stimulation using the restraint method. Then the rats were subjected to 3, 6, 12, 18 h of stress and 12, 24, 48, 72, 96 h of lymph node, spleen extracts and serum , And concanavalin A were added to the normal rat mesenteric lymph node cell suspension 72 h after incubation with melatonin (MTT) colorimetric assay melanocyte lymph node transformation (OD value), to observe the stress Inhibit the emergence and disappearance of lymphocyte transformation. The results were as follows: (1) No significant effect of lymph node, spleen extract and serum on the conversion of lymphocytes was observed in 3 and 6 h stress rats; (2) The expression of lymph node and spleen extract and Serum showed a significant inhibition of lymphocyte transformation, the role of stress 18h stronger; (3) lymph node and spleen extracts on lymphocyte transformation continued until 96 h after restraint release is still strong, but serum pairs The inhibitory effect of lymphocyte transformation gradually decreased after the restraint was released, and the effect disappeared after 96 h. The above results show that the rat restraint stress more than 12 h, the lymph node, spleen and serum simultaneously appear on lymphocyte transformation inhibition; this inhibition even after the restraint can still exist for a long time; inhibitory effect of serum time Shorter than lymph node and spleen inhibition time. The results suggest that restraint stress can cause lymphoid tissue to produce a kind of immune suppression of immune suppression protein (ISPS), and released into the blood