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目的:本实验研究灵芝孢子粉对戊四氮活化大鼠海马神经细胞caspase-9表达变化的影响,进一步探索灵芝孢子粉对癫痫大鼠脑保护的作用机制和癫痫与海马神经细胞凋亡调控基因之间的关系。方法:通过制备癫痫模型,RT-PCR和Western blotting检测正常对照组、癫痫模型组和灵芝孢子粉用药组caspase-9的表达。结果:癫痫模型组和灵芝孢子粉用药组caspase-9的表达较正常对照组均升高;其中癫痫模型组caspase-9的表达水平与对照组比较明显升高,灵芝孢子粉用药组caspase-9的表达水平与癫痫模型组比较明显降低,差异有统计学意义。结论:本研究结果证实,Caspase-9介导了癫痫大鼠神经细胞凋亡机制,说明灵芝孢子粉有效成份能充分作用于脑组织,可以调控caspase-9的表达,发挥抗凋亡的神经保护作用。
Objective: To study the effect of Ganoderma lucidum spore powder on the expression of caspase-9 in rat hippocampal neurons activated by pentylenetetrazole and to explore the mechanism of the protective effect of Ganoderma lucidum spore powder on the brain of epileptic rats and to explore the mechanism of epilepsy and the apoptosis- The relationship between. Methods: The expression of caspase-9 in normal control group, epilepsy model group and Ganoderma lucidum spore powder group was detected by preparing epilepsy model, RT-PCR and Western blotting. Results: The expression of caspase-9 in epileptic model group and Ganoderma lucidum spore powder group was higher than that in normal control group. The expression of caspase-9 in epilepsy model group was significantly higher than that in control group The expression level of epilepsy model group was significantly lower, the difference was statistically significant. CONCLUSIONS: The results of this study confirm that Caspase-9 mediates neuronal apoptosis in epileptic rats, indicating that active ingredients of Ganoderma lucidum spore powder can fully act on brain tissue and can regulate the expression of caspase-9 and exert anti-apoptotic neuroprotection effect.