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本文报道了一种新的BrdU给药法,即将BrdU溶解于溶融的琼脂液中,然后以一次皮下注射给予动物。与现已有方法相比,本法具有简单、快速,不需特殊设备,以及对动物损伤小和不必专门固定动物等优点。方法:用去离子水配制5％的琼脂Difco Bacto),在高压蒸锅中18磅120℃20分钟灭菌,冷却后保存在4℃下。临用前在沸水浴中融化,融化时将瓶塞拧松,然后将琼脂液(约80℃)倒入刻度试管中,并加入BrdU粉,强烈
In this paper, a new BrdU administration method is reported in which BrdU is dissolved in a molten agar solution and then given to the animal as a single subcutaneous injection. Compared with the existing methods, this method is simple, rapid, without special equipment, as well as small damage to animals and do not have to specifically fixed animals and so on. Method: 5% agar Difco Bacto in deionized water), sterilized in a pressure cooker at 18 pounds at 120 ° C for 20 minutes, and cooled at 4 ° C. Before use, thaw in a boiling water bath, unscrew the stopper when melted, then pour the agar solution (about 80 ° C) into a graduated test tube and add BrdU powder, strongly