β-1,4-半乳糖基转移酶Ⅱ、Ⅴ表达的亚细胞结构定位研究

• 作 者: 沈爱国 何江虹 丁斐 朱敏 王汉洲 顾建新
• 机 构: 南通医学院江苏省神经再生重点实验室,南通医学院江苏省神经再生重点实验室,南通医学院江苏省神经再生重点实验室,南通医学院江苏省神经再生重点实验室,复旦大学上海医学院基因研究中心,复旦大学上海医学院基因研
• 出 处: 神经解剖学杂志
• 发表日期: 2003年03期

为了研究β-1,4-半乳糖基转移酶 和 (β-1,4-Gal T- and )蛋白表达的亚细胞结构定位 ,本实验构建了β-1,4-Gal T- 和 融合绿色荧光蛋白 (GFP)表达质粒 ,分别将构建的质粒转染到 PC12细胞和肝癌 772 1细胞中 ,在荧光显微镜下观察β-1,4-Gal T- 和 在其中表达的亚细胞结构定位。发现 ,β-1,4-Gal T- 和 主要表达在这两种细胞的细胞核旁的 Golgi复合体 ,说明它们主要分布在 Golgi复合体上。提示它们可能是在 Golgi复合体参与蛋白质的糖链修饰

In order to study the subcellular localization of β-1,4-galactosyltransferase and (β-1,4-Gal T-and) protein expression, we constructed β-1,4-Gal T- Fluorescent protein (GFP) expression plasmids were constructed. The constructed plasmids were transfected into PC12 cells and hepatocellular carcinoma 772 1 cells respectively. The localization of β-1,4-Gal T- and the expression of subcellular structures therein was observed under a fluorescence microscope. It was found that [beta] -1, 4-Gal T- and Golgi complexes that are mainly expressed near the nucleus of both cells indicate that they are mainly distributed in the Golgi complex. Suggesting that they may be involved in the Golgi complex sugar chain modification of the protein