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研究晶状体疾病的实验方法有多种,在离体实验中,除了晶状体上皮细胞培养外,晶状体组织培养作为一种评价疾病发病风险和探讨病因的方法,在科研领域已有应用.尤其是在药物评价方面,此类培养方法可以前瞻性和回顾性地评价药物导致晶状体疾病发生的风险,筛选合适的药物.此外,通过晶状体体外培养构建的各类模型能够更加直观地反映晶状体的病理生理状态,对于晶状体周围环境的控制也更加方便.然而,晶状体组织培养的取材、培养、鉴定等方法不尽相同,本文从晶状体的生物学特性、取材、组织培养、状态鉴定及晶状体组织培养的应用几方面就近年来国内外有关晶状体体外培养的研究情况进行综述.“,”There are various methods to study lens disorders,mainly in vivo and in vitro.In vitro,besides lens epithelial cells culture,lens tissue culture has been widely used,which helps to evaluate onset risk and explore pathogenesis in scientific research field.Especially in pharmacology,lens tissue culture has manifested its superiority as a screening test that helps to estimate both ocular toxic and protective effects of a certain drug and screen out appropriate drug prospectively and retrospectively.In addition,models constructed by tissue culture reveal pathophysiology of lens more intuitively and their culture conditions are much easier to control.However,methods for sampling,culture and identification were diverse.Therefore,this review aimed to retrospect the front research of lens tissue culture both at home and aboard in biological property,sampling,tissue culture,identification of condition and application.