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目的：研究胃癌及正常胃粘膜ｃ－ｍｙｃ基因外显子ⅢＣＣＧＧ位点的甲基化状态及其ｍＲＮＡ和蛋白产物ｐ６７的表达。方法：应用Ｓｏｕｔｈｅｍｂｌｏｔ和原位杂交观察１４例胃癌和正常胃粘膜ｃ－ｍｙｃ基因ＤＮＡ甲基化与ｍＲＮＡ表达及ＳＡＢＣ法检测胃癌４５例、胃溃疡１４例、不典型增生１０例ｐ６７表达。结果：胃癌ｃ－ｍｙｃ基因外显子ⅢＣＣＧＧ位点比其相应正常胃粘膜呈高度去甲基化，ｍＲＮＡ表达升高（Ｐ＜０．００１），ＤＮＡ去甲基化与ｍＲＮＡ表达存在相关性（Ｐ＜０．０２５）。ｐ６７在胃癌、不典型增生、胃溃疡的表达率分别为８４．４４％（３８／４５）、１００％（１０／１０）、２１．４３％（３／１４），前两者与后者之间有显著性差异（Ｐ＜０．００１）。结论：ｃ－ｍｙｃ基因外显子ⅢＣＣＧＧ位点去甲基化及其表达产物可能与胃癌的发生发展有关。
OBJECTIVE: To investigate the methylation status of the exon III CCGG in c-myc gene and expression of mRNA and protein product p67 in gastric cancer and normal gastric mucosa. METHODS: DNA methylation and mRNA expression of c-myc gene were detected in 14 cases of gastric cancer and normal gastric mucosa by Southern blot and in situ hybridization. 45 cases of gastric cancer, 14 cases of gastric ulcer and 10 cases of atypical hyperplasia were detected by SABC method. RESULTS: The c-myc exon III CCGG of gastric cancer was highly demethylated and its mRNA expression was significantly higher than that of the corresponding normal gastric mucosa (P<0.001). There was a correlation between DNA demethylation and mRNA expression. P<0.025). The expression rates of p67 in gastric cancer, atypical hyperplasia, and gastric ulcer were 84.44% (38/45), 100% (10/10), and 21.43% (3/14), respectively. There was a significant difference (P<0.001). Conclusion: The demethylation of c-myc gene exon IIICCGG and its expression products may be related to the occurrence and development of gastric cancer.