论文部分内容阅读
目的 观察脑出血过程中血肿周边脑区谷氨酸 (Glu)、谷氨酸受体 (GluR)的变化规律 ,探讨其对局部神经元的损伤机制。方法 应用高效液相色谱、受体的放射配基结合分析等 ,监测实验性脑出血后大鼠血肿周边脑区的谷氨酸及其受体含量。结果 脑出血后 3h血肿周边脑区Glu含量开始升高 ,在血肿形成的高峰期 12h达峰值 ;而血肿周边脑区GluR的表达在脑出血后 6h处于明显的低表达状态 ;Glu的含量与GluR的表达呈显著的负相关 ;血肿周边脑区GluRKd值处于持续升高状态 ,且GluR与其Kd值变化表现非常显著的负相关。结论 Glu与血肿周边脑区神经元损伤密切相关 ,血肿周边脑区Glu与GluR结合力的分离 ,是血肿周边脑区神经元不可逆损伤的重要因素之一。
Objective To observe the changes of glutamate (Glu) and glutamate receptors (GluR) in the perihematomal brain regions during the intracerebral hemorrhage (ICH), and to explore the mechanism of local neuronal damage. Methods The content of glutamate and its receptor in peripheral brain regions of hematoma of rats after experimental intracerebral hemorrhage were detected by high performance liquid chromatography and radiolabeled ligand binding assay. Results The content of Glu in brain around the hematoma began to increase at 3h after intracerebral hemorrhage and peaked at 12h after the onset of hematoma. However, the expression of GluR in the brain around the hematoma was markedly low at 6h after intracerebral hemorrhage. There was a significant negative correlation between the expression of GluRKd in peripheral brain regions of hematoma and the significant negative correlation between GluR and Kd. Conclusion Glu is closely related to the neuronal damage in the perihematomal brain regions. The separation of Glu and GluR in the brain regions around the hematoma is one of the most important factors in the irreversible damage of the neurons in the perihematomal brain regions.