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目的 :建立复方马钱子片中士的宁和马钱子碱的含量测定方法。方法 :采用高效液相色谱法 ,以甲醇 乙腈 水 冰醋酸 (3 5∶40∶2 5∶0 .5 )为流动相 ,ZorbaxSB C18ODS(4 .6mm× 2 5 0 .0mm ,5 μm)为分析柱 ,紫外检测波长为 2 5 4nm。 结果 :士的宁线性范围为 0 .0 5～ 0 .45 μg ,r =0 .9997,测量平均回收率 99.4% ,RSD 2 .2 %。马钱子碱线性范围 0 .0 6～ 0 .5 4μg ,r =0 .9994,测量平均回收率 10 1.3 % ,RSD 1.2 2 %。结论 :高效液相色谱法测定复方马钱子片中士的宁和马钱子碱含量简便、快速、灵敏
Objective : To establish a method for the determination of the content of strychnine and strychnine in Compound Maqinzi Tablets. Methods: The HPLC method was used. The mobile phase was methanol acetonitrile glacial acetic acid (3∶40∶2 5∶0.5), ZorbaxSB C18ODS (4.6mm×25.0mm, 5 μm) as the analysis. Column, UV detection wavelength of 2 5 4nm. Results: The linear range of strychnine was 0.5 to 0.55 μg, r=0.9997. Mean recovery was 99.4% and RSD was 2.2%. The linear range of strychnine ranged from 0.06 to 0.54 μg, with r = .9994. Mean recovery was measured as 101.3% and RSD 1.2 2%. Conclusion :The content of strychnine and strychnine in compound Maqinzi Tablets is simple, fast, and sensitive by high performance liquid chromatography