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目的了解伯氏疟原虫(Plasmodium berghei NK65,P.b NK65)感染幼年和中年C57BL/6小鼠免疫反应特点。方法 P.b NK65感染3周龄幼年和8月龄中年C57BL/6小鼠,采用流式细胞分析技术检测不同年龄组小鼠脾细胞悬液中髓样树突状细胞(dendritic cells,DCs)数量,表面表达MHCⅡ类分子和CD86分子的DCs数量以及分泌IgG抗体的B细胞数量;ELISA方法检测脾细胞培养上清中IFN-γ和IL-4水平。结果 3周小鼠DCs数量[(40.08±3.54)×105]在感染后5 d明显升高,8月龄鼠在感染后5 d[(103.47±11.07)×105]及8 d[(104.04±1.92)×105]明显升高;3周鼠表面表达MHCⅡ类分子和CD86分子的DCs数量在感染后明显升高,8月龄鼠在感染后8 d表达MHCⅡ类分子[(239.40±16.32)×105]和CD86分子[(148.48±14.84)×105]数量明显高于3周鼠(P<0.05);3周鼠[(251.01±34.02)pg/mL]和8月龄鼠[(584.17±54.03)pg/mL]IFN-γ水平在感染后5 d均明显升高,2组比较差异有统计学意义(P<0.05)。结论 3周和8月龄C57BL/6小鼠感染P.b NK65后,其细胞免疫和体液免疫存在明显差异。
Objective To understand the immune response of Plasmodium berghei NK65 and P.b NK65 infected young and middle aged C57BL / 6 mice. Methods Pb NK65 was used to infect 3-week-old and 8-month-old middle-aged C57BL / 6 mice. Flow cytometry was used to detect the number of dendritic cells (DCs) in splenocyte suspension of different age groups , The number of DCs expressing MHC class II molecules and CD86 molecules, and the number of B cells secreting IgG antibodies. The levels of IFN-γ and IL-4 in the culture supernatant of splenocytes were detected by ELISA. Results The number of DCs in 3-week-old mice [(40.08 ± 3.54) × 105] was significantly increased at 5 days post-infection and was significantly higher at 8-day post- 1.92) × 105]. The number of DCs expressing MHC class II molecules and CD86 molecules on the 3rd week was significantly increased after infection. The MHC class II molecules [(239.40 ± 16.32) × (251.01 ± 34.02) pg / mL] and 8-month-old mice [(584.17 ± 54.03 ) pg / mL] IFN-γ levels were significantly increased 5 days after infection, the difference between the two groups was statistically significant (P <0.05). Conclusions There is a significant difference between C57BL / 6 mice infected with P.b NK65 at 3 weeks and 8 months after cell immunization and humoral immunity.