Immunohistochemical study of Fas antigen expression in synovial tissues from patients with rheumatoi

• 作 者: Yu Qinghong Liu Xiangyuan Shi
• 机 构: Department of Rheumatology, General Hospital of PLA, Beijing 100853, China
• 出 处: 中华医学杂志英文版
• 发表日期: 1998年3期
• 关键词: rheumatoid arthritis synovial tissue cell membrane Fas antigen

Objective To investigate the location and expression of Fas (Apo-1, CD-95) antigen in synovial tissue from rheumatoid arthritis (RA).Methods Immunohistochemical technique was used to identify the location and expression of Fas antigen in synovial tissues from 27 RA, 11 osteoarthritis (OA), 7 ankloysing spondylitis (AS), 3 pigmented villo-hyperplastic synovitis, 1 juvenile rheumatoid (JRA) patients and 5 normal control subjects.Results Fas was strongly expressed by synoviocytes and infiltrated lymphocytes in approximately two-thirds of RA patients (16/27). However, only weak expression occurred on lymphocytes in 3 of 11 OA, 1 of 7 AS patients and 1 of 5 normal subjects. The stain-positive substance in the forms of rings, granules, or dust was deposited on the cell membrane and in cytoplasm.Conclusion The expression of Fas may be involved in the mechanism of synovium proliferation and abnormal activation of local lymphocytes in RA.