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妊娠金黄地鼠随机分为实验组和对照组 ,前者于受精后第 8天置 42℃水浴中持续 2 0min ,后者置 37℃水浴中持续 2 0min。分别于高温后 8、12、2 4、48h剖腹取胎 ,石蜡包埋 ,连续切片。应用生物素标记的花生凝集素、刀豆凝集素、麦胚凝集和荆豆凝集素为分子探针作亲合细胞化学染色 ,对各期鼠胚神经上皮及其基膜、脊索和神经管周围间充质中相应受体糖基进行定性、定位和半定量观察。结果显示 ,正常胚胎神经上皮及其周围组织的花生凝集素、刀豆凝集素和麦胚凝集素均呈阳性着色 ,而荆豆凝集素在各个发育阶段均为阴性。高温致畸胚胎各胚龄组的凝集素着色与正常胚胎比较 ,花生凝集素和刀豆凝集素着色呈不同程度的减弱 ,麦胚凝集素着色无显著性差异 ,荆豆凝集素仍为阴性。上述结果表明 ,高温可导致神经上皮及其周围组织细胞表面某些糖基发生改变 ,破坏了细胞之间的正常粘着 ,这是高温致神经管畸形的一个重要途径。
Pregnant golden hamsters were randomly divided into experimental group and control group. The former was kept in 42 ℃ water bath for 20 min on the 8th day after fertilization, and the latter in the 37 ℃ water bath for 20 min. Respectively 8,12,2 4,48h after taking the cesarean section fetus, embedded in paraffin, serial sections. Biotin-labeled peanut lectin, polygonum bean agglutinin, wheat germ agglutination, and bean curd agglutinin were used as molecular probes for affinity cell chemiluminescence staining of embryonic neural epithelium and its basement membrane, spinal cord and neural tube Mesenchymal glycosylation of the corresponding receptor for qualitative, positioning and semi-quantitative observation. The results showed that peanut agglutinin, lactic bean agglutinin and wheat germ agglutinin in the normal embryonic nerve epithelium and its surrounding tissues were all positively stained, whereas the lentil was negative in all developmental stages. Compared with the normal embryos, the lectin of peanut lectin and polygonatum lectin decreased to different extents, while the lectin of wheat germ lectin had no significant difference, but lectin still remained negative. The above results show that high temperature can lead to some glycosylation on the surface of nerve epithelium and its surrounding tissue cells, which destroy the normal adhesion between cells, which is an important way of neural tube defects caused by hyperthermia.