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目的研究原发性小鼠新型隐球菌皮肤感染后产生的血行播散,检验皮肤作为隐球菌系统感染入侵的可能。方法新型隐球菌标准野生株B3501与ATCC32609分别皮内接种于环磷酰胺免疫抑制与非抑制的Balb/C小鼠,每组12只,待产生皮肤损伤后,分别于感染后1、2、4周处死,肝、胰、肾、脑、心、血液分别进行真菌培养,血清进行隐球菌荚膜抗原乳胶凝集试验。结果接种菌悬液后各组小鼠的接种部位形成皮肤损伤。环磷酰胺免疫抑制组和非抑制组皮肤损伤形成时间分别为3.42 d和4.25 d(P>0.05);皮肤损伤愈合时间分别为36.8 d和29.0 d(P<0.05)。在皮肤损伤形成后1、2、4周,各脏器和血液的真菌培养均为阴性。B3501、ATCC32609环磷酰胺处理组血清乳胶凝集试验阳性率分别为50%与17%,差异无统计学意义(P>0.05)。结论原发性皮肤隐球菌感染可能会导致免疫抑制的Balb/C小鼠血行播散。支持皮肤可能是隐球菌侵入机体的通道之一。
Objective To study the hematogenous dissemination of primary Cryptococcus neoformans skin infection and to test the possibility of invasion of the skin as a cryptococcal infection. Methods Cryptococcus neoformans standard wild strain B3501 and ATCC 32609 were inoculated intradermally in cyclophosphamide immunosuppressive and noninhibiting Balb / C mice, with 12 mice in each group. After skin injury was induced, Fight weeks, liver, pancreas, kidney, brain, heart, blood were cultured fungi, serum capsular antigen cryptococcosis test agglutination. Results The inoculation site of mice in each group formed skin lesions after inoculation of bacterial suspension. The formation time of skin lesions in cyclophosphamide immunosuppressive group and non-inhibiting group were 3.42 d and 4.25 d, respectively (P> 0.05). The wound healing time was 36.8 d and 29.0 d respectively (P <0.05). In the 1, 2, 4 weeks after the formation of skin lesions, all organ and blood fungal cultures were negative. The positive rate of serum latex agglutination test of B3501 and ATCC32609 treated groups was 50% and 17%, respectively, with no significant difference (P> 0.05). Conclusions Primary cutaneous cryptococcal infection may cause blood-borne Balb / C mice immunosuppressed. Supporting the skin may be one of the channels through which cryptococcosis invades the body.