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目的观察抗坏血酸(VC)对氯化锂诱导的腹腔巨噬细胞氧化损伤的保护作用。方法采用体外细胞培养法测定在VC(10、20、40、60、80、100μg//L)和氯化锂(50μg//L)作用下,测定体外VC和氯化锂对腹腔巨噬细胞的细胞抑制率、NO、H2O2及O2-指标。结果在各剂量VC和氯化锂作用下,巨噬细胞吞噬功能受到明显抑制,细胞抑制率分别为83.9%、87.5%、92.2%、96.9%,且呈现剂量-反应关系。巨噬细胞产生NO、H2O2及O2-的含量也受到明显抑制,呈现剂量-反应关系。结论 VC对巨噬细胞的吞噬功能,NO、H2O2及O2-等生物分子的产生均有明显促进作用,因此认为VC可影响巨噬细胞的非特异性防御功能。
Objective To observe the protective effect of ascorbic acid (VC) on oxidative damage of peritoneal macrophages induced by lithium chloride. Methods The in vitro cell culture method was used to determine the effects of VC and lithium chloride on peritoneal macrophages in vitro under the action of VC (10, 20, 40, 60, 80 and 100 μg / L) and lithium chloride Cell inhibition rate, NO, H2O2 and O2-indicators. Results The phagocytic function of macrophages was significantly inhibited under the action of VC and lithium chloride. The inhibitory rates of macrophages were 83.9%, 87.5%, 92.2% and 96.9%, respectively, and showed a dose-response relationship. The levels of NO, H2O2 and O2- produced by macrophages were also significantly inhibited, showing a dose-response relationship. Conclusions VC can significantly promote the phagocytosis of macrophages and the production of NO, H2O2 and O2-. Therefore, VC can affect the nonspecific defensive function of macrophages.